MECHANISM OF HOST INVASION BY SCHISTOSOMES

血吸虫宿主入侵机制

• 批准号: 8363596
• 负责人: James H. McKerrow
• 金额: $ 2.29万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363596
• 关键词: Classification; Drug Delivery Systems; Exposure to; Funding; Grant; Human; Imagery; Immune; Incubated; Informatics; Larva; Methods; National Center for Research Resources; Peptide Hydrolases; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Schistosoma; Schistosoma japonicum; Schistosoma mansoni; Schistosomiasis; Sequence Analysis; Skin; Source; Structural Models; United States National Institutes of Health; Work; biocomputing; cost; protein protein interaction; secretory protein; vaccine candidate

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project is focused on understanding the interaction between human and schistosoma proteins, with the aim of identifying drug targets and/or vaccine candidates for protection from schistosomiasis. Previous work on this project has been to analyze active secretory proteins from Schistosoma mansoni during initial host exposure to invasive larvae as a method for identifying functional proteins involved in invasion and host immune evasion. This work has expanded to analyze the the natural substrates for the proteases of invasive schistosome larvae in skin, where Schistosome cercariae are incubated with sections of human skin and products of degradation extracted. In a parallel track, the active proteases across multiple species including Schistosoma mansoni, Schistosoma japonicum and Schistosomatium douthitti were characterized for proteolytic classification and activity. For these projects, we use CGL resources for structural modeling and sequence analysis of the proteolytic enzymes. Recently we have added Cytoscape for network visualization of protein-protein interactions.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该项目的重点是了解人类和血吸虫蛋白之间的相互作用，目的是确定保护血吸虫病的药物靶点和/或候选疫苗。 该项目以前的工作一直是分析曼氏血吸虫在初始宿主暴露于入侵幼虫期间的活性分泌蛋白，作为鉴定参与入侵和宿主免疫逃避的功能蛋白的方法。这项工作已经扩展到分析皮肤中侵入性血吸虫幼虫蛋白酶的天然底物，其中血吸虫尾蚴与人类皮肤切片一起孵育，并提取降解产物。 在一个平行的轨道上，活性蛋白酶跨多个物种，包括血吸虫曼氏血吸虫，日本血吸虫和杜氏血吸虫的蛋白水解分类和活性的特点。 对于这些项目，我们使用CGL资源进行蛋白水解酶的结构建模和序列分析。 最近，我们添加了Cytoscape用于蛋白质-蛋白质相互作用的网络可视化。