SIRNA NANOPARTICLES
SIRNA纳米粒子
基本信息
- 批准号:8168579
- 负责人:
- 金额:$ 0.65万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-01-15 至 2010-12-31
- 项目状态:已结题
- 来源:
- 关键词:AminoglycosidesBehaviorCategoriesComplexComputer Retrieval of Information on Scientific Projects DatabaseFundingGene SilencingGoalsGrantHome environmentImageInstitutionLipidsNaturePhaseResearchResearch PersonnelResourcesRoentgen RaysSmall Interfering RNASourceStructureSystemUnited States National Institutes of Healthnanoparticleresearch study
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Complexes comprising siRNA and lipidic aminoglycoside derivatives have been recently imaged by Cryo-EM, displaying a typical layered microstructure.
In our lab we prepared lipid-siRNA constructs using a variety of lipid systems which have been successfully employed in gene silencing activities. The lipid-siRNA phase behavior appears to be very rich and so far we have identified by X-ray scattering three categories of supramolecular structures. Specifically: i) a lamellar phase obtained with commercially available cationic/non-ionic lipids, ii) a 2D hexagonal structure obtained with multivalent cationic lipids synthesized in our lab, and finally iii) a bicontinuous cubic phase obtained by a combination of a "cubic forming" neutral lipid and "home synthesized" multivalent cationic lipids (as well as commercially available). In addition, we have observed that the nature of the phase has a great impact on SE.
We propose Cryo-EM experiments to obtain 2D images of the lipid nanoparticles alone and upon aggregation with siRNA. Our long-term goals are to locate the siRNA domains in the lipid-siRNA aggregate.
这个子项目是众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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CYRUS R SAFINYA其他文献
CYRUS R SAFINYA的其他文献
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{{ truncateString('CYRUS R SAFINYA', 18)}}的其他基金
Functionalized Lipid Carriers for Nucleic-Acid and Drug Therapeutics
用于核酸和药物治疗的功能化脂质载体
- 批准号:
10020420 - 财政年份:2018
- 资助金额:
$ 0.65万 - 项目类别:
Functionalized Lipid Carriers for Nucleic-Acid and Drug Therapeutics
用于核酸和药物治疗的功能化脂质载体
- 批准号:
9789048 - 财政年份:2018
- 资助金额:
$ 0.65万 - 项目类别:
Functionalized Lipid Carriers for Nucleic-Acid and Drug Therapeutics
用于核酸和药物治疗的功能化脂质载体
- 批准号:
10242074 - 财政年份:2018
- 资助金额:
$ 0.65万 - 项目类别:
STRETCHING THE LIMITS OF MEMBRANE CHARGE DENSITY USING DENDRIMER LIPIDS
使用树枝状脂质扩展膜电荷密度的极限
- 批准号:
8362446 - 财政年份:2011
- 资助金额:
$ 0.65万 - 项目类别:
SYNCHROTRON X-RAY STUDIES OF BIOMOLECULAR MATERIALS
生物分子材料的同步辐射 X 射线研究
- 批准号:
8362233 - 财政年份:2011
- 资助金额:
$ 0.65万 - 项目类别:
STERICALLY STABILIZED CATIONIC LIPID - DNA COMPLEXES USED IN GENE DELIVERY
空间稳定的阳离子脂质 - 用于基因传递的 DNA 复合物
- 批准号:
8362475 - 财政年份:2011
- 资助金额:
$ 0.65万 - 项目类别:
STRETCHING THE LIMITS OF MEMBRANE CHARGE DENSITY USING DENDRIMER LIPIDS
使用树枝状脂质扩展膜电荷密度的极限
- 批准号:
8169665 - 财政年份:2010
- 资助金额:
$ 0.65万 - 项目类别:
SYNCHROTRON X-RAY STUDIES OF BIOMOLECULAR MATERIALS
生物分子材料的同步辐射 X 射线研究
- 批准号:
8169977 - 财政年份:2010
- 资助金额:
$ 0.65万 - 项目类别:
SYNCHROTRON X-RAY STUDIES OF BIOMOLECULAR MATERIALS
生物分子材料的同步辐射 X 射线研究
- 批准号:
8170193 - 财政年份:2010
- 资助金额:
$ 0.65万 - 项目类别:
STRETCHING THE LIMITS OF MEMBRANE CHARGE DENSITY USING DENDRIMER LIPIDS
使用树枝状脂质扩展膜电荷密度的极限
- 批准号:
7956428 - 财政年份:2009
- 资助金额:
$ 0.65万 - 项目类别:
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