SENSORY ION CHANNELS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 TRP家族通道的结构和功能。 我们已经使用cyo-EM和单粒子分析来确定TRP家族的离子通道的结构。 这些研究包括TRPV 1，疼痛热的受体，也感觉到辣椒中的活性成分，辣椒素，TRPV 2，另一种热传感器，以及TRPY 1，酵母液泡的离子通道，释放钙到细胞质中响应渗透压。 我们还使用cryo-EM来表征TRP通道被重建的囊泡，这使我们能够量化重建通道的活性。 非选择性阳离子通道的TRP家族代表了人体生理学中最重要的一类离子通道，并且构成了主要的一类药物靶标。 我们的工作代表了第一个可靠的三维结构信息，可用于任何全长TRP通道，也是第一个定量分析纯化和重建的TRP通道。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structure and function of TRP family channels. We have used cyo-EM and single particle analysis to determine structures of ion channels of the TRP family. Those studied included TRPV1, the receptor for painful heat that also senses the active ingredient in hot chili peppers, capsaicin, TRPV2, another heat sensor, and TRPY1, an ion channel of the yeast vacuole that releases calcium into the cytoplasm in response to osmotic stress. We have also used cryo-EM to characterize vesicles into which TRP channels been reconstituted, which allowed us to quantify the activity of the reconstituted channels. The TRP family of non-selective cation channels represent one of the most important classes of ion channels for human physiology, and constitutes a major class of drug targets. Our work represents the first reliable three-dimensional structural information available for any full-length TRP chanels, and also the first quantitative assays of purified and reconstituted TRP channels.