SENSORY ION CHANNELS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们已经使用cyo-EM和单粒子分析来确定离子通道的结构， TRP家族 这些研究包括TRPV 1，疼痛热的受体，也感觉 辣椒中的活性成分，辣椒素，TRPV 2，另一种热传感器，和TRPY 1， 酵母液泡的一种离子通道，响应于 渗透胁迫 我们还使用冷冻电镜来表征TRP通道进入的囊泡 这使我们能够量化重组通道的活性。 TRP家族的非选择性阳离子通道代表了最重要的一类 的离子通道，并构成了一个主要类别的药物靶标。 我们 这项工作代表了第一个可靠的三维结构信息， 全长TRP通道，也是第一个定量分析纯化和重建 TRP频道

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have used cyo-EM and single particle analysis to determine structures of ion channels of the TRP family. Those studied included TRPV1, the receptor for painful heat that also senses the active ingredient in hot chili peppers, capsaicin, TRPV2, another heat sensor, and TRPY1, an ion channel of the yeast vacuole that releases calcium into the cytoplasm in response to osmotic stress. We have also used cryo-EM to characterize vesicles into which TRP channels been reconstituted, which allowed us to quantify the activity of the reconstituted channels. The TRP family of non-selective cation channels represent one of the most important classes of ion channels for human physiology, and constitutes a major class of drug targets. Our work represents the first reliable three-dimensional structural information available for any full-length TRP chanels, and also the first quantitative assays of purified and reconstituted TRP channels.