LDL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 血浆低密度脂蛋白-胆固醇水平升高是动脉粥样硬化性冠状动脉疾病的主要危险因素(Gotto和Pownall，1999)。低密度脂蛋白由胆固醇酯(CES)和甘油三酯组成的中性脂肪核心，周围环绕着由磷脂、游离胆固醇和载脂蛋白B-100(apoB-100)组成的表面单层，载脂蛋白B-100包含启动低密度脂蛋白内吞的低密度脂蛋白受体(LDLR)的配体，低密度脂蛋白内吞是胆固醇代谢的关键步骤。如果我们能确定低密度脂蛋白/低密度脂蛋白受体复合体的结构，就能更好地了解低密度脂蛋白内吞作用的分子基础。我们对确定低密度脂蛋白和低密度脂蛋白受体复合体的低温EM结构感兴趣。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An elevated plasma level of low density lipoprotein (LDL)-cholesterol is a major risk factor for atherosclerotic coronary artery disease (Gotto and Pownall, 1999). LDL comprises a neutral lipid core of cholesteryl esters (CEs) and triglycerides, surrounded by a surface monolayer of phospholipids, free cholesterol, and apolipopro-tein B-100 (apoB-100), which contains the ligand for the LDL-receptor (LDLR) that initiates LDL endocytosis, a key step in cholesterol metabolism. The molecular basis of LDL endocytosis would be better understood if we could determine the structure of the LDL/LDLR complex. We are interested in determining the cryo-EM structure of the LDL and LDLR complex.