LDL

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 血浆低密度脂蛋白（LDL）-胆固醇水平升高是动脉粥样硬化性冠状动脉疾病的主要危险因素（Gotto和Pownall，1999）。LDL由胆固醇酯（CE）和甘油三酯组成的中性脂质核心组成，被磷脂、游离胆固醇和载脂蛋白B-100（apo B-100）的表面单层包围，载脂蛋白B-100含有LDL受体（LDLR）的配体，可启动LDL内吞作用，这是胆固醇代谢的关键步骤。如果我们能确定LDL/LDLR复合物的结构，将更好地理解LDL内吞作用的分子基础。我们有兴趣确定LDL和LDLR复合物的冷冻电镜结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An elevated plasma level of low density lipoprotein (LDL)-cholesterol is a major risk factor for atherosclerotic coronary artery disease (Gotto and Pownall, 1999). LDL comprises a neutral lipid core of cholesteryl esters (CEs) and triglycerides, surrounded by a surface monolayer of phospholipids, free cholesterol, and apolipopro-tein B-100 (apoB-100), which contains the ligand for the LDL-receptor (LDLR) that initiates LDL endocytosis, a key step in cholesterol metabolism. The molecular basis of LDL endocytosis would be better understood if we could determine the structure of the LDL/LDLR complex. We are interested in determining the cryo-EM structure of the LDL and LDLR complex.