LDL

低密度脂蛋白

• 批准号: 8168530
• 负责人: Henry J. Pownall
• 金额: $ 2.15万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168530
• 关键词: Cholesterol; Cholesterol Esters; Cholesterol Homeostasis; Complex; Computer Retrieval of Information on Scientific Projects Database; Coronary Arteriosclerosis; Endocytosis; Funding; Grant; Institution; LDL Cholesterol Lipoproteins; Ligands; Lipids; Low Density Lipoprotein Receptor; Low-Density Lipoproteins; Molecular; Phospholipids; Plasma; Research; Research Personnel; Resources; Risk Factors; Source; Structure; Surface; Triglycerides; United States National Institutes of Health; apolipoprotein B-100; base; interest; monolayer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An elevated plasma level of low density lipoprotein (LDL)-cholesterol is a major risk factor for atherosclerotic coronary artery disease (Gotto and Pownall, 1999). LDL comprises a neutral lipid core of cholesteryl esters (CEs) and triglycerides, surrounded by a surface monolayer of phospholipids, free cholesterol, and apolipopro-tein B-100 (apoB-100), which contains the ligand for the LDL-receptor (LDLR) that initiates LDL endocytosis, a key step in cholesterol metabolism. The molecular basis of LDL endocytosis would be better understood if we could determine the structure of the LDL/LDLR complex. We are interested in determining the cryo-EM structure of the LDL and LDLR complex.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 血浆低密度脂蛋白（LDL）水平升高是动脉粥样硬化性冠状动脉疾病的主要危险因素（Gotto 和 Pownall，1999）。 LDL 包含胆固醇酯 (CE) 和甘油三酯的中性脂质核心，周围是磷脂、游离胆固醇和载脂蛋白 B-100 (apoB-100) 的表面单层，其中载脂蛋白 B-100 (apoB-100) 含有 LDL 受体 (LDLR) 的配体，可启动 LDL 内吞作用，这是胆固醇的关键步骤 新陈代谢。如果我们能够确定 LDL/LDLR 复合物的结构，就能更好地理解 LDL 内吞作用的分子基础。我们有兴趣确定 LDL 和 LDLR 复合物的冷冻电镜结构。