HDL WITH SOF

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 人血浆高密度脂蛋白(HDL)，逆转胆固醇的主要载体 转运，是血清不透明因子(SOF)的靶标，SOF是一种毒力决定因素 使血清变得不透明的化脓性链球菌。高密度脂蛋白由中性脂质组成的核心， 胆固醇酯和一些甘油三酯，被胆固醇的表面单层包围， 磷脂和特殊蛋白质[载脂蛋白(Apos)A-I和A-II]。高密度脂蛋白是一种 驻留在动能陷阱中的不稳定粒子，它可以通过杂化、洗涤剂、 或者是热扰动。重组(R)SOF催化几乎所有中性的转移 脂类由~100,000个高密度脂蛋白颗粒(D~8.5 nm)转变为单一的、大的富含胆固醇的酯 微乳液(Cerm；D&gt；100 nm)，留下新的类高密度脂蛋白颗粒[neo高密度脂蛋白(D~5.8 NM)]，同时释放无脂(Lf)载脂蛋白A-I。CEM的形成和apo A-I的释放相似 动力学的，指平行的或快速的连续的步骤。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Human plasma high-density lipoproteins (HDL), the primary vehicle for reverse cholesterol transport, are the target of serum opacity factor (SOF), a virulence determinant of Streptococcus pyogenes that turns serum opaque. HDL comprises a core of neutral lipids, cholesteryl esters and some triglycerides, surrounded by a surface monolayer of cholesterol, phospholipids, and specialized proteins [apolipoproteins (apos) A-I and A-II]. A HDL is an unstable particle residing in a kinetic trap from which it can escape via chaotropic, detergent, or thermal perturbation. Recombinant (r) SOF catalyzes the transfer of nearly all neutral lipids of ~100,000 HDL particles (D ~ 8.5 nm) into a single, large cholesteryl ester-rich microemulsion (CERM; D > 100 nm), leaving a new HDL-like particle [neo HDL (D ~ 5.8 nm)] while releasing lipid-free (LF) apo A-I. CERM formation and apo A-I release have similar kinetics, suggesting parallel or rapid consecutive steps.