HDL WITH SOF

HDL 与 SOF

• 批准号: 8361103
• 负责人: Henry J. Pownall
• 金额: $ 0.49万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361103
• 关键词: Apolipoprotein A-I; Cholesterol; Cholesterol Esters; Detergents; Electron Microscopy; Funding; Grant; High Density Lipoproteins; Human; Kinetics; Left; Lipids; National Center for Research Resources; Phospholipids; Plasma; Principal Investigator; Proteins; Recombinants; Research; Research Infrastructure; Resources; Serum; Source; Streptococcus pyogenes; Surface; Triglycerides; United States National Institutes of Health; Virulence; apolipoprotein D; cost; macromolecule; monolayer; opacity factor; particle; reverse cholesterol transport

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Human plasma high-density lipoproteins (HDL), the primary vehicle for reverse cholesterol transport, are the target of serum opacity factor (SOF), a virulence determinant of Streptococcus pyogenes that turns serum opaque. HDL comprises a core of neutral lipids, cholesteryl esters and some triglycerides, surrounded by a surface monolayer of cholesterol, phospholipids, and specialized proteins [apolipoproteins (apos) A-I and A-II]. A HDL is an unstable particle residing in a kinetic trap from which it can escape via chaotropic, detergent, or thermal perturbation. Recombinant (r) SOF catalyzes the transfer of nearly all neutral lipids of ~100,000 HDL particles (D ~ 8.5 nm) into a single, large cholesteryl ester-rich microemulsion (CERM; D > 100 nm), leaving a new HDL-like particle [neo HDL (D ~ 5.8 nm)] while releasing lipid-free (LF) apo A-I. CERM formation and apo A-I release have similar kinetics, suggesting parallel or rapid consecutive steps.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 人血浆高密度脂蛋白（HDL），逆转胆固醇的主要载体 运输，是血清混浊因子（SOF）的目标，血清混浊因子是一种毒力决定因素， 使血清不透明的化脓性链球菌。HDL包含中性脂质的核心， 胆固醇酯和一些甘油三酯，被胆固醇的表面单层包围， 磷脂和特化蛋白质[载脂蛋白（apo）A-I和A-II]。HDL是一种 存在于动力学阱中的不稳定颗粒，它可以通过离液序列高的，去污剂， 或热扰动。重组（r）SOF催化几乎所有中性的 约100，000 HDL颗粒（D约8.5 nm）的脂质转化为单个大的富含胆固醇酯的 微乳液（CERM; D > 100 nm），留下新的HDL样颗粒[neo HDL（D ~ 5.8 nm）]，同时释放无脂质（LF）apo A-I。CERM的形成和apo A-I的释放具有相似的 动力学，表明平行或快速连续的步骤。