LDL

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 血浆低密度脂蛋白-胆固醇水平升高是动脉粥样硬化性冠状动脉疾病的主要危险因素(Gotto和Pownall，1999)。低密度脂蛋白由胆固醇酯(CES)和甘油三酯组成的中性脂肪核心，周围环绕着由磷脂、游离胆固醇和载脂蛋白B-100(apoB-100)组成的表面单层，载脂蛋白B-100包含启动低密度脂蛋白内吞的低密度脂蛋白受体(LDLR)的配体，低密度脂蛋白内吞是胆固醇代谢的关键步骤。如果我们能确定低密度脂蛋白/低密度脂蛋白受体复合体的结构，就能更好地了解低密度脂蛋白内吞作用的分子基础。我们对确定低密度脂蛋白和低密度脂蛋白受体复合体的低温EM结构感兴趣。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. An elevated plasma level of low density lipoprotein (LDL)-cholesterol is a major risk factor for atherosclerotic coronary artery disease (Gotto and Pownall, 1999). LDL comprises a neutral lipid core of cholesteryl esters (CEs) and triglycerides, surrounded by a surface monolayer of phospholipids, free cholesterol, and apolipopro-tein B-100 (apoB-100), which contains the ligand for the LDL-receptor (LDLR) that initiates LDL endocytosis, a key step in cholesterol metabolism. The molecular basis of LDL endocytosis would be better understood if we could determine the structure of the LDL/LDLR complex. We are interested in determining the cryo-EM structure of the LDL and LDLR complex.