LDL

低密度脂蛋白

• 批准号: 8361060
• 负责人: Henry J. Pownall
• 金额: $ 1.23万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361060
• 关键词: Cholesterol; Cholesterol Esters; Cholesterol Homeostasis; Complex; Coronary Arteriosclerosis; Electron Microscopy; Endocytosis; Funding; Grant; LDL Cholesterol Lipoproteins; Ligands; Lipids; Low Density Lipoprotein Receptor; Low-Density Lipoproteins; Molecular; National Center for Research Resources; Phospholipids; Plasma; Principal Investigator; Research; Research Infrastructure; Resources; Risk Factors; Source; Structure; Surface; Triglycerides; United States National Institutes of Health; base; cost; interest; macromolecule; monolayer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. An elevated plasma level of low density lipoprotein (LDL)-cholesterol is a major risk factor for atherosclerotic coronary artery disease (Gotto and Pownall, 1999). LDL comprises a neutral lipid core of cholesteryl esters (CEs) and triglycerides, surrounded by a surface monolayer of phospholipids, free cholesterol, and apolipopro-tein B-100 (apoB-100), which contains the ligand for the LDL-receptor (LDLR) that initiates LDL endocytosis, a key step in cholesterol metabolism. The molecular basis of LDL endocytosis would be better understood if we could determine the structure of the LDL/LDLR complex. We are interested in determining the cryo-EM structure of the LDL and LDLR complex.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 血浆低密度脂蛋白（LDL）-胆固醇水平升高是动脉粥样硬化性冠状动脉疾病的主要危险因素（Gotto和Pownall，1999）。LDL由胆固醇酯（CE）和甘油三酯组成的中性脂质核心组成，被磷脂、游离胆固醇和载脂蛋白B-100（apo B-100）的表面单层包围，载脂蛋白B-100含有LDL受体（LDLR）的配体，可启动LDL内吞作用，这是胆固醇代谢的关键步骤。如果我们能确定LDL/LDLR复合物的结构，将更好地理解LDL内吞作用的分子基础。我们有兴趣确定LDL和LDLR复合物的冷冻电镜结构。