HDL WITH SOF

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 链球菌血清不透明因子(SOF)是一种使血清浑浊的毒力决定因素，会导致高密度脂蛋白不稳定。SOF似乎催化了~100,000个高密度脂蛋白颗粒(D~90)的CE转移到一个大的富含CE的微乳液中 (Cerm；R&gt；1,000？)留下一种新的类高密度脂蛋白颗粒，新的高密度脂蛋白(D~20？)，并释放无脂(LF)载脂蛋白A-I。RSOF是一种异二价融合蛋白，它利用一个对接位点取代载脂蛋白A-I，并与高密度脂蛋白上暴露的CE表面结合；由此产生的rSOF-高密度脂蛋白复合体利用其结合脱脂位点招募额外的高密度脂蛋白，并通过多个融合步骤形成CEIM。我们希望通过Cryo-EM来证实这种对SOF活性的解释，并发现更多关于neo-高密度脂蛋白和CERM的信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Streptococcal serum opacity factor (SOF), a virulence determinant that clouds serum, causes HDL instability. SOF appears to catalyzes transfer of the CE of ~100,000 HDL particles (D ~ 90 ¿) into a large CE-rich microemulsion (CERM; r >1,000 ¿) leaving a new HDL-like particle, neo HDL (D ~ 20¿), and releasing lipid-free(LF) apo A-I. rSOF is a heterodivalent fusogenic protein that uses a docking site to displace apo A-I and bind to exposed CE surfaces on HDL; the resulting rSOF-HDL complex recruits additional HDL with its binding delipidation site and, through multiple fusion steps, forms a CERM. By cryo-EM we hope to confirm this interpretation of SOF activity, and uncover more information about neo-HDL and CERMs.