HDL WITH SOF

HDL 与 SOF

• 批准号: 8168595
• 负责人: Henry J. Pownall
• 金额: $ 2.15万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168595
• 关键词: Apolipoprotein A-I; Apolipoproteins; Cholesterol; Cholesterol Esters; Computer Retrieval of Information on Scientific Projects Database; Detergents; Funding; Grant; High Density Lipoproteins; Human; Institution; Kinetics; Left; Lipids; Phospholipids; Plasma; Proteins; Recombinants; Research; Research Personnel; Resources; Serum; Source; Streptococcus pyogenes; Surface; Triglycerides; United States National Institutes of Health; Virulence; apolipoprotein D; monolayer; opacity factor; particle; reverse cholesterol transport

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human plasma high-density lipoproteins (HDL), the primary vehicle for reverse cholesterol transport, are the target of serum opacity factor (SOF), a virulence determinant of Streptococcus pyogenes that turns serum opaque. HDL comprises a core of neutral lipids, cholesteryl esters and some triglycerides, surrounded by a surface monolayer of cholesterol, phospholipids, and specialized proteins [apolipoproteins (apos) A-I and A-II]. A HDL is an unstable particle residing in a kinetic trap from which it can escape via chaotropic, detergent, or thermal perturbation. Recombinant (r) SOF catalyzes the transfer of nearly all neutral lipids of ?100,000 HDL particles (D ? 8.5 nm) into a single, large cholesteryl ester-rich microemulsion (CERM; D > 100 nm), leaving a new HDL-like particle [neo HDL (D ? 5.8 nm)] while releasing lipid-free (LF) apo A-I. CERM formation and apo A-I release have similar kinetics, suggesting parallel or rapid consecutive steps.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 人血浆高密度脂蛋白(HDL)是胆固醇反向运输的主要载体，是血清不透明因子(SOF)的靶标，SOF是化脓性链球菌的毒力决定因素，可使血清变得不透明。高密度脂蛋白由中性脂质、胆固醇酯和一些甘油三酯组成，周围环绕着胆固醇、磷脂和特殊蛋白质[载脂蛋白(Apos)A-I和A-II]的表面单层。高密度脂蛋白是一种驻留在动能陷阱中的不稳定粒子，它可以通过杂化、洗涤剂或热扰动逃逸。重组(R)SOF催化将100,000个高密度脂蛋白颗粒(D？8.5 nm)的几乎所有中性脂转移到一个大的富含胆固醇酯的微乳液(Cem；D&gt；100 nm)中，留下一个新的高密度脂蛋白样颗粒[neo HDL(D？5.8 nm)]，同时释放无脂(LF)载脂蛋白A-I。CEM的形成和apo A-I的释放具有相似的动力学，意味着平行或快速的连续步骤。