INTEGRATIVE APPROACH TO CORTICAL COGNITIVE NETWORKS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个项目有两个主要目标。第一个是确定工作记忆过程中联合皮质(前额叶和后顶叶)中认知神经元网络的结构和功能特性。第二个目标在很大程度上是方法论的：证实工作记忆中神经活动和血流动力学变化之间的耦合。在猴子身上，这两个目标将通过组合使用四种微创和行为兼容的记录方法来实现：近红外光谱(NIRS)、表面场电位(FP)记录、局部场电位(LFP)记录和单位活动记录。近红外信号和表面FP将用硬膜外探头同时记录。单位活动和局部场电位(LFP)也将通过跨硬膜微电极同时记录。基于认知网络结构的某些假设和每种方法的空间分辨率，这四种方法将被联合使用来检验两个工作记忆任务执行期间感兴趣区域的神经激活和血流动力学变化的三个具体假设，即空间延迟响应(DR)和非空间延迟样本匹配(DMS)。分析将集中于在工作记忆中保留感觉刺激期间的神经和血液动力学活动。NIR、FP和单元数据将与与要测试的特定假设最相关的每个变量相关联：皮质位置、刺激或备忘录、任务、试验时间和正确表现水平。在认知功能的神经-血流动力学耦合的研究中，将特别强调近红外信号与细胞放电的电表现之间的相关性。这些相关性有望为人类认知中功能成像信号的神经元基础提供关键信息，例如通过BOLD fMRI获得的信号。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project has two major objectives. The first is to identify structural and functional properties of cognitive neuronal networks in cortex of association (prefrontal and posterior parietal) during working memory. The second objective is largely methodological: to substantiate the coupling between neural activity and hemodynamic changes in working memory. Both objectives will be pursued in the monkey by the combined use of four minimally invasive and behavior-compatible recording methods: near-infrared spectroscopy (NIRS), surface field-potential (FP) recording, local field-potential (LFP) recording, and unit-activity recording. NIRS signals and surface FPs will be recorded simultaneously with epidural probes. Unit activity and local field potentials (LFPs) will also be recorded simultaneously by means of transdural microelectrodes. Based on certain assumptions of cognitive network architecture and the spatial resolution of each method, the four methods will be used in combination to test three specific hypotheses of neural activation and hemodynamic change in the regions of interest during the performance of two working-memory tasks, spatial delayed response (DR) and non-spatial delayed matching to sample (DMS). The analysis will focus on the neural and hemodynamic activity during the retention of a sensory stimulus in working memory. NIRS, FP, and unit data will be correlated with each of the variables most relevant to the specific hypotheses to be tested: cortical location, stimulus or memorandum, task, time of trial, and level of correct performance. In the study of neural-hemodynamic coupling in cognitive function, special emphasis will be placed on the correlations between NIRS signals and electrical manifestations of cell discharge. These correlations are expected to provide crucial information on the neuronal basis of functional imaging signals, such as those obtained by BOLD fMRI, in human cognition.