PROTEIN ENGINEERING OF BIRA FOR PROTEIN TAGGING

用于蛋白质标记的 BIRA 蛋白质工程

• 批准号: 8169295
• 负责人: CATHERINE L DRENNAN
• 金额: $ 0.41万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169295
• 关键词: Binding; Computer Retrieval of Information on Scientific Projects Database; E coli birA protein; Engineering; Funding; Grant; Institution; Mediating; Methods; Protein Engineering; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; X-Ray Crystallography; improved

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To improve the efficiency of an E. coli Biotin Ligase (BirA) mediated protein tagging method, efforts are being made to increase the promiscuity of BirA by protein engineering of its binding pocket. Our contribution toward these engineering efforts uses X-ray crystallography to study the contacts between BirA and its substrates.

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