课题基金 / 基金详情

PROTEIN ENGINEERING OF BIRA FOR PROTEIN TAGGING

PROTEIN ENGINEERING OF BIRA FOR PROTEIN TAGGING
用于蛋白质标记的 BIRA 蛋白质工程
批准号：
8169295
负责人：
CATHERINE L DRENNAN
金额：
$0.41万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
项目状态：
已结题
起止时间：
2010-04-01 至 2011-03-31
来源：
https://reporter.nih.gov/project-details/8169295
关键词：
BindingComputer Retrieval of Information on Scientific Projects DatabaseE coli birA proteinEngineeringFundingGrantInstitutionMediatingMethodsProtein EngineeringProteinsResearchResearch PersonnelResourcesSourceUnited States National Institutes of HealthX-Ray Crystallographyimproved

项目摘要

项目成果

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中文摘要
翻译
这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为了提高大肠杆菌生物素连接酶(BIRA)介导的蛋白质标记方法的效率，人们正在努力通过对BIRA结合口袋进行蛋白质工程来增加BIRA的混杂程度。我们对这些工程工作的贡献是使用X射线结晶学来研究Bira与其底物之间的接触。
英文摘要
This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To improve the efficiency of an E. coli Biotin Ligase (BirA) mediated protein tagging method, efforts are being made to increase the promiscuity of BirA by protein engineering of its binding pocket. Our contribution toward these engineering efforts uses X-ray crystallography to study the contacts between BirA and its substrates.
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