COMPLEX OF CORRINOID IRON-SULFUR PROTEIN AND ITS METHYLTRANSFERASE

咕啉铁硫蛋白复合物及其甲基转移酶

• 批准号: 8169291
• 负责人: CATHERINE L DRENNAN
• 金额: $ 0.41万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169291
• 关键词: Binding; Complex; Computer Retrieval of Information on Scientific Projects Database; Corrinoids; Funding; Grant; Hand; Institution; Iron-Sulfur Proteins; Light; Methyltransferase; Pathway interactions; Proteins; Reaction; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; Wood material; carbon fixation; methyl group; protein structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A key step in the Wood-Ljungdahl carbon fixation pathway is the transfer of a methyl group from methyltetrahydrofolate, bound by a methyltransferase (MeTr), to the corrinoid ring of the corrinoid ironsulfur protein (CFeSP). Separate crystal structures of MeTr and CFeSP have not been sufficient in explaining how the methyl transfer reaction takes place; therefore, we seek to shed light on this methyl hand-off by obtaining a crystal structure of these proteins in complex.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Wood-Ljungdahl碳固定途径中的关键步骤是通过甲基转移酶（MeTr）将甲基从甲基四氢叶酸转移到类咕啉铁的类咕啉环硫蛋白（CFeSP）。MeTr和CFeSP的单独晶体结构不足以解释甲基转移反应是如何发生的;因此，我们试图通过获得这些蛋白质的晶体结构来阐明这种甲基转移。