COMPLEX OF CORRINOID IRON-SULFUR PROTEIN AND ITS METHYLTRANSFERASE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Wood-Ljugdahl固碳途径中的一个关键步骤是甲基四氢叶酸的甲基转移酶(Metr)结合到皮质铁硫蛋白(CFeSP)的皮质环上。Metr和CFeSP单独的晶体结构不足以解释甲基转移反应是如何发生的；因此，我们试图通过获得这些蛋白质在复合体中的晶体结构来阐明这种甲基转移。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A key step in the Wood-Ljungdahl carbon fixation pathway is the transfer of a methyl group from methyltetrahydrofolate, bound by a methyltransferase (MeTr), to the corrinoid ring of the corrinoid ironsulfur protein (CFeSP). Separate crystal structures of MeTr and CFeSP have not been sufficient in explaining how the methyl transfer reaction takes place; therefore, we seek to shed light on this methyl hand-off by obtaining a crystal structure of these proteins in complex.