ACTIN-DEPENDENT ENDOCYTOSLS IN BUDDING YEAST

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在酵母细胞中，内吞作用完全依赖于肌动蛋白细胞骨架。我们目前研究的一个主要领域是阐明肌动蛋白的内吞作用。我们已经分离出了大量的突变株，它们在这个过程中的不同步骤阻止了酵母的内吞作用，我们还用GFP标记了大约20-30个内吞和细胞骨架蛋白。我们将使用X射线断层摄影术来充分阐明内吞途径的空间组织，并将这一途径与各种其他细胞特征联系起来。我们将确定这些特征在细胞周期中如何变化，以响应内吞作用对Ste2p受体蛋白内吞作用的反应，以及对特定内吞和细胞骨架蛋白突变的响应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In yeast cells, endocytosis is entirely dependent upon the actin cytoskeleton. A major current area of our research is to elucidate the endocytic roles of actin. We have isolated numerous mutants that block endocytosis in yeast at discrete steps in the process, and we tagged some 20-30 endocytic and cytoskeletal proteins with GFP. We will use x-ray tomography to fully elucidate the spatial organization of the endocytic pathway and to relate this pathway with a variety of other cellular features. We will determine how these features change through the cell cycle in response to endocytic induction of the Ste2p receptor protein endocytosis and in response to mutation of specific endocytic and cytoskeletal proteins.