ACTIN-DEPENDENT ENDOCYTOSLS IN BUDDING YEAST

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在酵母细胞中，内吞作用完全依赖于肌动蛋白细胞骨架。我们目前的一个主要研究领域是阐明肌动蛋白的内吞作用。我们已经分离出了许多突变体，在酵母中阻断内吞作用的离散步骤的过程中，我们标记了一些20-30内吞和细胞骨架蛋白与GFP。我们将使用X射线断层扫描来充分阐明内吞途径的空间组织，并将此途径与其他各种细胞特征联系起来。我们将确定这些功能如何通过细胞周期的变化，在响应内吞诱导的Ste 2 p受体蛋白内吞和响应特定的内吞和细胞骨架蛋白的突变。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In yeast cells, endocytosis is entirely dependent upon the actin cytoskeleton. A major current area of our research is to elucidate the endocytic roles of actin. We have isolated numerous mutants that block endocytosis in yeast at discrete steps in the process, and we tagged some 20-30 endocytic and cytoskeletal proteins with GFP. We will use x-ray tomography to fully elucidate the spatial organization of the endocytic pathway and to relate this pathway with a variety of other cellular features. We will determine how these features change through the cell cycle in response to endocytic induction of the Ste2p receptor protein endocytosis and in response to mutation of specific endocytic and cytoskeletal proteins.