课题基金 / 基金详情

IDENTIFICATION OF KINETOCHORE INTERACTING PROTEINS (KNL-1/KNL-3/KNL-2)

IDENTIFICATION OF KINETOCHORE INTERACTING PROTEINS (KNL-1/KNL-3/KNL-2)
动粒相互作用蛋白的鉴定 (KNL-1/KNL-3/KNL-2)
批准号:
8171385
负责人:
Arshad Desai
金额:
$0.24万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2010
资助国家:
美国
项目状态:
已结题
起止时间:
2010-09-01 至 2011-08-31

项目摘要

项目成果

Arshad Desai的其他基金

相似基金

相关文献

中文摘要
翻译
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Polo-like kinase 1 (Plk1) is a central mitotic kinase implicated in the regulation of the mitotic checkpoint, a mechanism controlling chromosomes segregation and preventing loss or gain of whole chromosomes, which is a known cause of birth defects and a contributing factor in the genesis of cancer. The multiplicity of Plk1 functions for has made it difficult to deconvolve its function in cell division. A potential approach to circumventing this problem is to identify kinetochore-specific PLK-1 substrates. We identified a series of N-terminal ?MELT? repeats in a protein called KNL-1 (Kinetochore Null protein-1) that fit the consensus site for PLK-1 phosphorylation. KNL-1 is a protein located on the chromosomes necessary for the assembly of the kinetochore and chromosomes segregation. We are testing the hypothesis that KNL-1 is a PLK-1 substrate and investigate the role of this phosphoregulation in chromosome segregation and checkpoint signaling. As predicted by the location of the MELT repeats, the N-terminal fragment of KNL-1 (KNL-11-505) is an excellent PLK-1 substrate in vitro (radioactive kinase assays). To assess the function of PLK-1 phosphorylation of KNL-1, in collaboration with the Scripps Research Institute Center for Physiological Proteomics, we mapped PLK-1 target sites on KNL-1 by using a combination of in vitro kinase assays and analysis by mass spectrometry. 6 phosphosites were each identified multiple times in the 3 samples we submitted to analysis, indicating that they are major sites on KNL-1 for PLK-1 phosphorylation. Now we are investigating the role of these phosphorylations in the control of mitosis progression and chromosomes segregation in C.elegans embryos.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Kinetochore Assembly and Regulation
Kinetochore Specification and Function
IDENTIFICATION OF INTERACTING PROTEINS OF SPINDLY
  • 批准号:
    8171402
  • 项目类别:
  • 资助金额:
    $0.24万
  • 财政年份:
    2010
  • 负责人:
    Arshad Desai
  • 依托单位:
IDENTIFICATION OF PHOSPHORYLATION SITES AURORA B
  • 批准号:
    8171401
  • 项目类别:
  • 资助金额:
    $0.24万
  • 财政年份:
    2010
  • 负责人:
    Arshad Desai
  • 依托单位:
国内基金
海外基金
犬钩虫中Caenorhabditis elegans daf同源基因的鉴定和功能研究
  • 批准号:
    30972181
  • 项目类别:
    面上项目
  • 资助金额:
    30.0万元
  • 批准年份:
    2009
  • 负责人:
    杨玉荣
  • 依托单位:
利用线虫(Caenorhabditis elegans)模型研究14-3-3蛋白在机体抵御逆境因子胁迫过程中的分子作用机制
  • 批准号:
    30771234
  • 项目类别:
    面上项目
  • 资助金额:
    30.0万元
  • 批准年份:
    2007
  • 负责人:
    王亚梅
  • 依托单位: