ANALYSIS OF CEN DNA-MICROTUBULE ATTACHMENT IN VITRO IN BUDDING YEAST

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 动点是纺锤体微管的主要染色体附着部位，在染色体分离中起着中心作用。酿酒酵母(S.cerevisiae)是研究着丝粒组装的理想模型，因为它有125个碱基的序列特异性着丝粒。到目前为止，已鉴定出60多个芽期酵母动粒蛋白，其中许多在高等生物中保守。然而，目前还不清楚这些蛋白质如何发挥功能来确保动粒-纺锤体的附着。野生型或突变酵母菌株的提取物将用于确定序列附着特异性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Kinetochores function as the primary chromosomal attachment sites for spindle microtubules and play a central role in chromosome segregation. S. cerevisiae is an ideal model in which to study kinetochore assembly due to its 125 base pair sequence-specific centromere. To date, over 60 budding yeast kinetochore proteins have been identified, many of which are conserved in higher organisms. Nevertheless, it is not clear how these proteins function to ensure kinetochore-spindle attachment. Extracts from wild-type or mutant yeast strains will be used to determine sequence attachment specificity.