ANALYSIS OF CEN DNA-MICROTUBULE ATTACHMENT IN VITRO IN BUDDING YEAST

芽殖酵母 CEN DNA-微管附着的体外分析

• 批准号: 7602213
• 负责人: Arshad Desai
• 金额: $ 0.08万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602213
• 关键词: Base Pairing; Centromere; Chromosome Segregation; Computer Retrieval of Information on Scientific Projects Database; Ensure; Funding; Grant; In Vitro; Institution; Kinetochores; Microtubules; Modeling; Organism; Play; Proteins; Research; Research Personnel; Resources; Role; Saccharomyces cerevisiae; Saccharomycetales; Site; Source; Specificity; United States National Institutes of Health; Yeasts; mutant; protein function

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Kinetochores function as the primary chromosomal attachment sites for spindle microtubules and play a central role in chromosome segregation. S. cerevisiae is an ideal model in which to study kinetochore assembly due to its 125 base pair sequence-specific centromere. To date, over 60 budding yeast kinetochore proteins have been identified, many of which are conserved in higher organisms. Nevertheless, it is not clear how these proteins function to ensure kinetochore-spindle attachment. Extracts from wild-type or mutant yeast strains will be used to determine sequence attachment specificity.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 动力学充当纺锤微管的主要染色体附着位点，并在染色体分离中起着核心作用。 S. cerevisiae是一个理想的模型，在该模型中，由于其125个碱基对序列特异性的丝粒，研究动力学组装。 迄今为止，已经鉴定出60多种萌芽的酵母菌蛋白，其中许多蛋白在较高的生物体中是保守的。 然而，尚不清楚这些蛋白质如何发挥作用以确保动力学主轴附着。野生型或突变酵母菌菌株的提取物将用于确定序列附着特异性。