ANALYSIS OF CEN DNA-MICROTUBULE ATTACHMENT IN VITRO IN BUDDING YEAST

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 着丝粒作为纺锤体微管的主要染色体附着位点，在染色体分离中起着重要作用。 S.酿酒酵母由于其125个碱基对的序列特异性着丝粒，是研究动粒组装的理想模型。 迄今为止，已经鉴定了60多种芽殖酵母动粒蛋白，其中许多在高等生物中是保守的。 然而，目前尚不清楚这些蛋白质如何发挥作用，以确保着丝粒纺锤体的附件。将使用野生型或突变酵母菌株的提取物测定序列连接特异性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Kinetochores function as the primary chromosomal attachment sites for spindle microtubules and play a central role in chromosome segregation. S. cerevisiae is an ideal model in which to study kinetochore assembly due to its 125 base pair sequence-specific centromere. To date, over 60 budding yeast kinetochore proteins have been identified, many of which are conserved in higher organisms. Nevertheless, it is not clear how these proteins function to ensure kinetochore-spindle attachment. Extracts from wild-type or mutant yeast strains will be used to determine sequence attachment specificity.