ANALYSIS OF CEN DNA-MICROTUBULE ATTACHMENT IN VITRO IN BUDDING YEAST

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Kinetochores function as the primary chromosomal attachment sites for spindle microtubules and play a central role in chromosome segregation. S. cerevisiae is an ideal model in which to study kinetochore assembly due to its 125 base pair sequence-specific centromere. To date, over 60 budding yeast kinetochore proteins have been identified, many of which are conserved in higher organisms. Nevertheless, it is not clear how these proteins function to ensure kinetochore-spindle attachment. Extracts from wild-type or mutant yeast strains will be used to determine sequence attachment specificity.