NACL/DNASEI/MNASE RELEASED PROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在先前发现不同的转录因子(TF)被释放之后 在不同的盐浓度下，我试图测试不同盐浓度的梯度 并查看每一步释放的TF的配置文件。此外，我试图复制一个 来自Henikoff et.的发现。艾尔基因组研究2009年表明，如果你治疗细胞核 用MNASe，然后进行盐分馏分，低盐馏分对应于 人类基因组中活跃的转录元件。不幸的是， 我用的DNA酶不足以消化细胞核，所以DNA酶数据类似于 盐度数据。 该项目提供了许多有趣的结果，目前正在使用SRM进行验证， CHIP-SEQ和SILAC类似的方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Subsequent to the previous findings that different transcription factors (TFs) are released with different salt concentrations, I sought to test a gradient of different salt concentrations and see the profile of TFs that are released by each step. Additionally, I sought to replicate a finding from Henikoff et. al. Genome Research 2009 that showed that if you treat nuclei with MNAse, and subsequently do salt fractionation, the low salt fractions correspond to active transcriptional elements in the human genome. Unfortunately, the concentration of DNAse that I used was not sufficient to digest the nuclei, so the DNAse data is analogous to the salt data. This project provided many interesting results that are currently being validated using SRM, ChIP-seq and SILAC like approaches.