IMPROVED METHOD TO LABEL PROTEINS WITH GFP

用 GFP 标记蛋白质的改进方法

• 批准号: 8171232
• 负责人: ERIC MULLER
• 金额: $ 8.43万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171232
• 关键词: Cellular biology; Cherry - dietary; Color; Computer Retrieval of Information on Scientific Projects Database; Fluorescence Resonance Energy Transfer; Funding; Grant; Institution; Label; Measures; Methods; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Variant; improved; in vivo

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability to tag proteins with GFP and its color variants has revolutionized cell biology. In particular the CFP/YFP pair and the GFP/Cherry pair of fluorescent proteins make excellent FRET partners to measure the proximity of two proteins in vivo. We plan to develop a new method to tag proteins with the GFP variants.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 用GFP及其颜色变体标记蛋白质的能力彻底改变了细胞生物学。特别是CFP/YFP对和GFP/Cherry对荧光蛋白是测量体内两种蛋白质接近度的优良FRET伴侣。 我们计划开发一种新的方法来标记具有GFP变体的蛋白质。