CONVERSION OF FRETR CATEGORIES TO DISTANCES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，但不一定是研究者所在的机构。 开发FRTR度量以测量CFP和YFP之间的FRET。它取代了通常是非线性的并且对低值不敏感的其他度量。FRET是相对的，经验的FRET测量。 它表示来自FRET的荧光相对于源自CFP和YFP的固有荧光性质的基线的倍数增加。 在我们对纺锤体极体（SPB）组织的研究中，FRETR值被分为5大类：无、最低、低、中等和高。我们的SBP SPB和冷冻EM图像模型限制了每类CFP和YFP之间的可能距离。本项目将进一步探索FRETR值与荧光团之间距离的关系。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The FRETR metric was developed to measure FRET between CFP and YFP. It replaces other metrics which generally are nonlinear and are insensitive to low values. FRETR is relative, empirical measure of FRET. It expresses the fold increase of fluorescence from FRET over a baseline derived from the inherent fluorescent properties of CFP and YFP. In our study on the organization of the spindle pole body (SPB), FRETR values were divided into 5 broad categories: none, lowest, low, moderate and high. Our model of the SPB and cryo-EM images of the SBP put limits on the possible distances between CFP and YFP for each class. This project will further explore the relationship between FRETR values and the distance between fluorophores.