CONVERSION OF FRETR CATEGORIES TO DISTANCES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 FRETR度量是用来测量CFP和YFP之间的FRET的。它取代了其他指标，这些指标通常是非线性的，对低值不敏感。FRETR是相对的、经验性的FRET衡量标准。它表示来自FRET的荧光在基线上的倍数增加，源于CFP和YFP的固有荧光特性。 在我们对纺锤极体(SPB)组织的研究中，FRETR值被分为5大类：无、最低、低、中和高。我们的SPB模型和SBP的低温EM图像限制了每个类别的CFP和YFP之间的可能距离。该项目将进一步探索FRETR值和荧光团之间的距离之间的关系。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The FRETR metric was developed to measure FRET between CFP and YFP. It replaces other metrics which generally are nonlinear and are insensitive to low values. FRETR is relative, empirical measure of FRET. It expresses the fold increase of fluorescence from FRET over a baseline derived from the inherent fluorescent properties of CFP and YFP. In our study on the organization of the spindle pole body (SPB), FRETR values were divided into 5 broad categories: none, lowest, low, moderate and high. Our model of the SPB and cryo-EM images of the SBP put limits on the possible distances between CFP and YFP for each class. This project will further explore the relationship between FRETR values and the distance between fluorophores.