IMPROVED METHOD TO LABEL PROTEINS WITH GFP

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 用绿色荧光蛋白及其颜色变体标记蛋白质的能力使细胞生物学发生了革命性的变化。特别是，CFP/YFP对和GFP/Cherry对荧光蛋白是测量体内两种蛋白质接近程度的极佳FRET合作伙伴。 我们计划开发一种新的方法来标记带有GFP变体的蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The ability to tag proteins with GFP and its color variants has revolutionized cell biology. In particular the CFP/YFP pair and the GFP/Cherry pair of fluorescent proteins make excellent FRET partners to measure the proximity of two proteins in vivo. We plan to develop a new method to tag proteins with the GFP variants.