IMPROVED PROTOCOL TO STAIN THE CELL SURFACE, ACTIN AND DNA OF YEAST

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 为了捕捉酵母菌株之间的广泛多样性的快照，我们选择检查总细胞形态。特别是，我们想检查细胞在细胞周期不同阶段的形状，以及潜在的肌动蛋白分布。目前公布的方法染色细胞表面和肌动蛋白细胞骨架既不稳健，也流线。因此，我们开发了新的协议。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to capture a snapshot of the broad diversity among yeast strains we chose to examine gross cell morphology. In particular we wanted to examine the shape of the cells at different phases of the cell cycle, and the underlying actin distribution. Current published methods for staining the cell surface and actin cytoskeleton were neither robust nor stream-lined. Thus we developed new protocols.