DISSEMINATION OF STRAINS AND PLASMIDS BY THE MICROSCOPY GROUP

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 显微镜组先前构建了携带CFP、YFP、Venus和 在酵母中表达良好的mCherry ORF。这些质粒允许荧光蛋白融合 通过基于PCR的整合将其连接到任何酵母蛋白的C末端。我们改变了一个质粒 由Prein等人设计，使得YFP或CFP可以通过 基于PCR的整合。 我们也有超过1000个酵母菌株含有标记的荧光 proteins.

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The microscopy group previously constructed plasmids carrying CFP, YFP, Venus, and mCherry ORFs that express well in yeast. These plasmids allow fusion of fluorescent proteins to the C-terminus of any yeast protein by PCR-based integration. We altered a plasmid designed by Prein et al. so that YFP or CFP can be fused to the N-terminus of proteins by PCR-based integration. We also have over 1000 yeast strains containing tagged fluorescent proteins.