TUB4 COMPLEX IN YEAST
酵母中的 TUB4 复合物
基本信息
- 批准号:8171267
- 负责人:
- 金额:$ 0.24万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-01 至 2011-08-31
- 项目状态:已结题
- 来源:
- 关键词:BaculovirusesCentrosomeCollaborationsComplexComputer Retrieval of Information on Scientific Projects DatabaseEukaryotic CellFundingGrantInstitutionMicrotubulesNuclearOrganellesProteinsResearchResearch PersonnelResourcesSourceUnited States National Institutes of HealthWorkYeastsgamma Tubulinreconstitutionspindle pole body
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
The yeast spindle pole body (SPB) is functionally equivalent to the centrosome in higher eukaryotic cells. The SPB is a multilayered organelle that organizes both the nuclear and cytoplasmic microtubules. We are working toward reconstitution of the microtubule nucleating activity of the SPB. The well characterized small gamma-tubulin complex contains three proteins: Tub4p, Spc97p, and Spc98p. We have expressed all three proteins in baculovirus and found that they readily assemble into a stable, soluble complex. However, the nucleation capacity of the small complex is quite low, suggesting we are missing components required for nucleation. The yeast spindle pole body (SPB) is functionally equivalent to the centrosome in higher eukaryotic cells. The SPB is a multilayered organelle that organizes both the nuclear and cytoplasmic microtubules. We are working toward reconstitution of the microtubule nucleating activity of the SPB. The well characterized small gamma-tubulin complex contains three proteins: Tub4p, Spc97p, and Spc98p. We have expressed all three proteins in baculovirus and found that they readily assemble into a stable, soluble complex. However, the nucleation capacity of the small complex is quite low. Recently the Agard lab (in collaboration with us) showed that the Tub4p molecules in the small complex are too far apart to nucleate microtubules.
这个子项目是许多研究子项目中利用
资源由NIH/NCRR资助的中心拨款提供。子项目和
调查员(PI)可能从NIH的另一个来源获得了主要资金,
并因此可以在其他清晰的条目中表示。列出的机构是
该中心不一定是调查人员的机构。
酵母纺锤体极体(SPB)在功能上等同于高等真核细胞中的中心体。SPB是一个多层细胞器,既有核的也有细胞质的微管。我们正在努力重建SPB的微管成核活性。鉴定良好的小分子微管蛋白复合体包含三种蛋白质:Tub4p、Spc97p和Spc98p。我们已经在杆状病毒中表达了这三种蛋白,并发现它们很容易组装成稳定的、可溶的复合体。然而,小复合体的成核能力相当低,这表明我们缺少成核所需的成分。酵母纺锤体极体(SPB)在功能上等同于高等真核细胞中的中心体。SPB是一个多层细胞器,既有核的也有细胞质的微管。我们正在努力重建SPB的微管成核活性。鉴定良好的小分子微管蛋白复合体包含三种蛋白质:Tub4p、Spc97p和Spc98p。我们已经在杆状病毒中表达了这三种蛋白,并发现它们很容易组装成稳定的、可溶的复合体。然而,小分子络合物的成核能力很低。最近,阿加德实验室(与我们合作)显示,小复合体中的Tub4p分子相距太远,无法形成微管。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Trisha N. Davis其他文献
A Bayesian Integrative Structure Model of the Yeast Centrosome
- DOI:
10.1016/j.bpj.2017.11.240 - 发表时间:
2018-02-02 - 期刊:
- 影响因子:
- 作者:
Shruthi Viswanath;Massimiliano Bonomi;Seung Joong Kim;Vadim A. Klenchin;Keenan Taylor;King C. Yabut;Neil T. Umbreit;Janet Meehl;Michele H. Jones;Javier Velazquez-Muriel;Mark Winey;Ivan Rayment;Trisha N. Davis;Andrej Sali;Eric D. Muller - 通讯作者:
Eric D. Muller
Design of a hyperstable 60-subunit protein icosahedron
一种超稳定的 60 亚基蛋白质二十面体的设计
- DOI:
10.1038/nature18010 - 发表时间:
2016-06-15 - 期刊:
- 影响因子:48.500
- 作者:
Yang Hsia;Jacob B. Bale;Shane Gonen;Dan Shi;William Sheffler;Kimberly K. Fong;Una Nattermann;Chunfu Xu;Po-Ssu Huang;Rashmi Ravichandran;Sue Yi;Trisha N. Davis;Tamir Gonen;Neil P. King;David Baker - 通讯作者:
David Baker
Reconstitution Of Microtubule-driven Movement and Force Production by the Ndc80 Kinetochore Complex
- DOI:
10.1016/j.bpj.2008.12.3744 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:
- 作者:
Andrew D. Franck;Andrew F. Powers;Daniel R. Gestaut;Jeremy Cooper;Beth Gracyzk;Ronnie R. Wei;Linda Wordeman;Trisha N. Davis;Charles L. Asbury - 通讯作者:
Charles L. Asbury
The spindle pole body of Saccharomyces cerevisiae: architecture and assembly of the core components.
酿酒酵母纺锤体杆体:核心部件的结构和组装。
- DOI:
10.1016/s0070-2153(99)49006-4 - 发表时间:
2000 - 期刊:
- 影响因子:0
- 作者:
Susan E. Francis;Trisha N. Davis - 通讯作者:
Trisha N. Davis
Genetic analysis of yeast spindle pole bodies.
酵母纺锤体极体的遗传分析。
- DOI:
10.1016/s0091-679x(01)67007-9 - 发表时间:
2001 - 期刊:
- 影响因子:0
- 作者:
Trisha N. Davis - 通讯作者:
Trisha N. Davis
Trisha N. Davis的其他文献
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{{ truncateString('Trisha N. Davis', 18)}}的其他基金
Comprhensive Biology: Exploiting the Yeast Genome
综合生物学:利用酵母基因组
- 批准号:
8416531 - 财政年份:2012
- 资助金额:
$ 0.24万 - 项目类别:
ISOTOPE SIGNATURE BASED IDENTIFICATION OF CROSSLINKED PEPTIDES BY MS
通过 MS 基于同位素特征的交联肽鉴定
- 批准号:
8171348 - 财政年份:2010
- 资助金额:
$ 0.24万 - 项目类别:
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