ANALYSIS OF PARTIALLY RECONSTITUTED KINETOCHORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 动粒是一种高度保守的复合体，负责在有丝分裂期间使微管与染色体之间附着。虽然动粒的主要子复合物已经被确定，但对这些子复合物的结构组织知之甚少。我们想确定哪些蛋白质/亚复合物在细菌中表达时是共纯化的，以帮助结构分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The kinetochore is a highly conserved complex responsible for making the attachment between microtubules and chromosomes during mitosis. Although the major sub-complexes of the kinetochore have been identified, relatively little is known about the structural organization of these sub-complexes. We would like to determine which proteins/sub-complexes are copurifying when expressed in bacteria to aid in structural analysis.