ADDITIONAL ATTEMPTS AT ENRICHING FOR TRANSCRIPTION FACTORS (VELOS)

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目由大量实验组成，这些实验旨在以选择性地从细胞核中富集转录因子的方式处理细胞核。大多数实验都是使用20世纪60年代至70年代的论文中的协议设计的。改变的条件包括：盐浓度、蔗糖浓度、EDTA/EGTA存在、蔗糖分级、亚精胺和DNA酶浓度。 这些实验的主要发现之一是盐浓度对转录因子回收率的影响最大。此外，观察到MCX柱未完全清除样品中的NP-40，这可能导致实验2中观察到的色谱污染。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is composed of a large number of experiments designed at treating nuclei in such a manner that transcription factors are selectively enriched from them. Most of the experiments were designed using protocols from papers in the 1960s-1970s. The conditions that were varied include: salt concentration, sucrose concentration, EDTA/EGTA presence, Sucrose fractionation, Spermidine and DNAse concentration. One of the main findings from these experiments was that the salt concentration had the largest effect on the transcription factor recovery. Additionally, it was observed that the MCX cartridges were not fully cleaning up the NP-40 from the samples, which was probably causing the chromatographic contamination that was observed in experiment 2.