STRUCTURAL STUDIES ON SULFATE TRANSPORTERS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Sat-1（Slc 26 a1）是一种钠非依赖性硫酸盐阴离子转运蛋白，作为阴离子交换剂。Sat-1是一种膜蛋白，在特定细胞中无机硫酸盐的细胞内摄取中起着至关重要的作用，然后用于各种代谢和生长过程。sat-1介导的硫酸盐重吸收在实验性慢性肾功能衰竭中的生理重要性已得到证实，这与肾脏sat-1表达的显著下调和硫酸盐排泄分数的显著增加有关。我们已经确定了这个家庭的多个细菌同源物，并建议确定Sat-1的结构，以了解其运输机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sat-1 (Slc26a1) is a sodium-independent sulfate anion transporter that operates as an anion exchanger. Sat-1 is a membrane protein that plays a crucial role in intracellular uptake of inorganic sulfate in specific cells, which is then utilized in various metabolic and growth processes. The physiological importance of the sat-1-mediated sulfate reabsorption has been confirmed in experimental chronic renal failure, which is associated with major downregulation of the renal sat-1 expression and significant increase of the fractional sulfate excretion. We have identified multiple bacterial homologues of this family and propose to determine the structure of Sat-1 in order to understand its transport mechanism.