STRUCTURAL STUDIES ON SULFATE TRANSPORTERS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 SAT-1(Slc26a1)是一种不依赖钠离子的硫酸盐阴离子转运体，作为阴离子交换器运行。SAT-1是一种膜蛋白，在特定细胞内对无机硫酸盐的吸收起着至关重要的作用，这些无机硫酸盐随后被用于各种代谢和生长过程。Sat-1介导的硫酸盐重吸收在实验性慢性肾功能衰竭中的生理重要性已被证实，这与肾脏Sat-1表达的显著下调和部分硫酸盐排泄的显著增加有关。我们已经鉴定了该家族的多种细菌同源物，并建议确定Sat-1的结构，以了解其转运机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sat-1 (Slc26a1) is a sodium-independent sulfate anion transporter that operates as an anion exchanger. Sat-1 is a membrane protein that plays a crucial role in intracellular uptake of inorganic sulfate in specific cells, which is then utilized in various metabolic and growth processes. The physiological importance of the sat-1-mediated sulfate reabsorption has been confirmed in experimental chronic renal failure, which is associated with major downregulation of the renal sat-1 expression and significant increase of the fractional sulfate excretion. We have identified multiple bacterial homologues of this family and propose to determine the structure of Sat-1 in order to understand its transport mechanism.