SIDE-VIEWING RAMAN OPTICAL FIBER PROBE FOR BIOMEDICAL SPECTROSCOPY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 我们正在研究使用拉曼光谱引导立体定向核心针活检的乳房可疑微钙化发现筛查乳腺X线摄影。微钙化是乳腺组织内钙矿物盐的沉积，其通过乳房X线摄影定位最具临床意义的异常，因此是随后针吸活检的目标。 拉曼光谱对含钙矿物质的存在非常敏感，因此可用于检测和定位乳房中的微钙化。这可以通过与现有针吸活检设备兼容的光纤探针以非破坏性的方式在体内实时完成。侧视拉曼光纤探头提供了利用来自拉曼源的激发光并在探头插入针装置时将光传递到乳房组织并从乳房组织收集光的能力。该探头设计为适合在立体定向空心针活检过程中最常用的9号针器械。与已成功用于体内和体外临床研究的前视拉曼探针类似，侧视探针结合了具有涂层的激发和发射滤光片，以抑制光纤背景并能够有效提取拉曼信号。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are investigating the usage of Raman spectroscopy to guide stereotactic core needle biopsies of the breast for suspicious microcalcifications discovered at screening mammography. Microcalcifications are deposits of calcium mineral salts within breast tissue that mammographically localize the most clinically significant abnormality and are, therefore, a target for subsequent needle biopsy. Raman spectroscopy is exquisitely sensitive to the presence of calcium-containing minerals, and thus can be used to detect and localize microcalcifications in the breast. This can be done in vivo, in real-time, in a non-destructive manner, via optical fiber probes that are compatible with existing needle biopsy devices. The side-viewing Raman fiber optic probe provides capability of utilizing the excitation light from Raman source and delivering to and collecting the light from the breast tissue while the probe inserted into needle device. The probe is designed to fit into 9 gauge needle device most commonly used during stereotactic core needle biopsies. Similar to the front-viewing Raman probe that has been successfully used in the in vivo and ex vivo clinical studies, the side-viewing probe incorporate excitation and emission filters with coatings to suppress fiber background and enable efficient extraction of the Raman signal.