MONOCLONAL ANTIBODY PRODUCTION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项研究的目的是在临床试验中产生抗体检测的抗血清。为了改进检测方法，以确定是否产生了抗体，需要制作阳性对照材料。该抗体的耐受性很好，到目前为止在人类中只有很少的抗Ab反应，没有产生足够的血清作为临床试验期间运行的所有分析的阳性对照。由于已知的抗体，以前使用的动物再次被使用，并每周接受皮下注射。当血清中存在足够多的抗体时(通过酶联免疫吸附试验技术测定)，收集到单次大容量出血。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this study was to produce antisera of an antibody testing in a clinical trial. To improve the assay to determine if anti-Abs are being produced, positive control material needed to be made. The antibody has been well tolerated, with only a few anti-Ab responses to date in human, not enough serum was produced to use as a positive control for all the assays run during the clinical trials. Previously used animal was used once again because of known antibodies and received weekly subcutaneous injections. When enough antibodies were present in the serum (as determined by ELISA techniques) a single large volume bleed was collected.