MONOCLONAL ANTIBODY PRODUCTION

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 本研究的目的是生产用于临床试验的抗体检测的抗血清。 为了改进测定以确定是否产生抗Ab，需要制备阳性对照材料。 该抗体具有良好的耐受性，迄今为止在人体中仅有少数抗Ab应答，未产生足够的血清用作临床试验期间运行的所有试验的阳性对照。由于已知抗体，再次使用先前使用的动物，并每周接受皮下注射。当血清中存在足够的抗体时（如通过ELISA技术测定的），收集单次大体积出血。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this study was to produce antisera of an antibody testing in a clinical trial. To improve the assay to determine if anti-Abs are being produced, positive control material needed to be made. The antibody has been well tolerated, with only a few anti-Ab responses to date in human, not enough serum was produced to use as a positive control for all the assays run during the clinical trials. Previously used animal was used once again because of known antibodies and received weekly subcutaneous injections. When enough antibodies were present in the serum (as determined by ELISA techniques) a single large volume bleed was collected.