MONOCLONAL ANTIBODY PRODUCTION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本研究的目的是生产用于临床试验的抗体检测的抗血清。 为了改进测定以确定是否产生抗Ab，需要制备阳性对照材料。 该抗体具有良好的耐受性，迄今为止在人体中仅有少数抗Ab应答，未产生足够的血清用作临床试验期间运行的所有试验的阳性对照。由于已知抗体，再次使用先前使用的动物，并每周接受皮下注射。当血清中存在足够的抗体时（如通过ELISA技术测定的），收集单次大体积出血。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this study was to produce antisera of an antibody testing in a clinical trial. To improve the assay to determine if anti-Abs are being produced, positive control material needed to be made. The antibody has been well tolerated, with only a few anti-Ab responses to date in human, not enough serum was produced to use as a positive control for all the assays run during the clinical trials. Previously used animal was used once again because of known antibodies and received weekly subcutaneous injections. When enough antibodies were present in the serum (as determined by ELISA techniques) a single large volume bleed was collected.