Pregnancy/NO Induced Changes in UAE Ca2+Signaling

怀孕/NO 引起的阿联酋 Ca2 信号变化

• 批准号: 8256751
• 负责人: IAN M. BIRD
• 金额: $ 36.74万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8256751
• 关键词: ATP Receptors; Abbreviations; Adult; Adverse effects; Affect; Agonist; Animal Model; Animals; Apamin; Arteries; Birth; Blood Pressure; Blood Vessels; Blood flow; Cell Count; Cell Line; Cell membrane; Cell physiology; Cells; Communication; Connexin 43; Connexins; Country; Coupled; Coupling; Data; Dependence; Diabetes Mellitus; Disease; Electrophysiology (science); Endothelial Cells; Endothelium; Event; Failure; Fetal Growth Retardation; Funding; Future; GTP-Binding Proteins; Gap Junctions; Growth Factor; Human; Image; In Vitro; Individual; Inositol; Ion Channel; Ions; Knowledge; Life; Measures; Mediating; Mediator of activation protein; Membrane; Membrane Potentials; Modeling; Mothers; Movement; Nitric Oxide; Obesity; One-Step dentin bonding system; P2X-receptor; Perfusion; Pharmaceutical Preparations; Phospholipase C; Phosphorylation; Placenta; Play; Population; Potassium Channel; Pre-Eclampsia; Pregnancy; Probability; Process; Production; Relative (related person); Resistance; Risk; Role; Series; Signal Transduction; Smooth Muscle Myocytes; TRPC3 ion channel; Testing; VEGF165; Vascular Endothelial Growth Factors; Vascular Endothelium; Vascular Smooth Muscle; Vasodilation; Woman; Work; density; functional restoration; human NOS3 protein; improved; in vivo; inhibitor/antagonist; myometrium; pregnant; pressure; public health relevance; receptor; response; success; therapeutic target; treatment planning; unborn child; voltage

DESCRIPTION (provided by applicant): Preeclampsia (PE) adversely affects as many as 20% of pregnancies in the USA. One of the earliest events is the failure of maternal vascular endothelium vasodilatory function to adapt, with the normal pregnancy-associated increase in NO being blunted or lost. In our previous funding period we used dual imaging of [Ca2+]i and NO in freshly isolated uterine artery endothelium to show that in normal pregnancy, enhancement of Ca2+ signaling is the primary cause of enhanced NO production, and such enhancement depends on specific changes in function of Ca2+ signaling mediators. Sustained Ca2+ entry into UA endothelium occurs through TRPC channels but our preliminary data also suggests the pregnancy enhanced opening of TRPC channels in turn depends on CX43 Gap junction communication, and that such coupling of cells may occur at an electrical level. Our data further suggests membrane hyperpolarization by KCa type channels connects increased Gap junction function to enhance TRPC function in pregnancy. Consistent with this, UAEC show sensitivity to antagonists of KCa function (Apamin and Tram34) with a resulting reduction in Ca2+ signaling. If this model is correct then future therapy for failed adaptation of Ca2+ signaling in PE pregnancy could be achieved by the use of SK or IK type KCa channel agonists. In this renewal we propose to test this model, moving from isolated cells in culture to whole vessels ex vivo to whole animal studies. We will examine 1) Changes in membrane potential and KCa current activity in response to ATP in NP- and P-UAEC in cells at low vs high density, dependence of these changes on gap junction function, and determine the effect of antagonists Apamin and Tram-34 or the selective agonist NS309 on Ca2+ burst activity to implicate KCa channels in this response; 2) Examine the effects of these same antagonists and agonists on changes in Ca2+, membrane potential and NO production in intact UA vessels ex vivo; 3) determine if the KCa agonist NS309 is comparable to the effects of ATP/UTP in mediating NO dependent increases in blood flow in uterine artery in vivo during pregnancy, and also the extent to which SK and/or IK types of KCa channel mediate this effect. In this way we take our recent advances in mechanistic knowledge of pregnancy adaptation of blood flow to now establish the validity of using KCa channel agonists to maximize this response in vivo in pregnancy and particularly those complicated by PE. Success could have major impact on strategies to improve treatment of human PE pregnancy where this otherwise normal adaptive response has failed and is resistant to current therapies. PUBLIC HEALTH RELEVANCE: Preeclampsia (PE) is a potentially devastating disease of pregnancy that can threaten the life of both mother and the unborn child. It affects as many as 20% of the population. Even mild PE is dangerous and can cause preterm (early) birth and this then gives the baby an increased risk to develop several diseases such as blood pressure, diabetes and obesity as an adult. It is believed that in PE the cells lining the uterine artery blood vessels fail to increase their function as would otherwise happen in normal pregnancy. We have evidence to suggest normal adaptation is due to electrical changes and their failure may be 'fixed' with a specific drug NS309. The aim of this project is to find out if our suspicions are correct by testing in an animal model. If we are right we may be able to come up with a treatment plan fro PE pregnancy. Having more complete knowledge of how cell function is regulated in pregnancy will certainly take us one step closer to overcoming this potentially devastating disease that effects so many in this country and beyond.

描述（由申请人提供）：先兆子痫（PE）对美国多达20%的妊娠产生不利影响。最早期的事件之一是母体血管内皮舒张功能不能适应，与正常妊娠相关的NO增加被钝化或丢失。在我们之前的资助期间，我们在新鲜分离的子宫动脉内皮中使用[Ca 2 +]i和NO的双重成像，以表明在正常妊娠中，Ca 2+信号的增强是NO产生增强的主要原因，并且这种增强取决于Ca 2+信号介质功能的特定变化。持续的Ca 2+进入UA内皮通过TRPC通道发生，但我们的初步数据也表明，妊娠增强TRPC通道的开放反过来取决于CX 43间隙连接通讯，这种细胞偶联可能发生在一个电水平。我们的数据进一步表明，KCa型通道的膜超极化与间隙连接功能的增加有关，从而增强了妊娠期TRPC的功能。与此一致，UAEC显示出对KCa功能拮抗剂（Apamin和Tram 34）的敏感性，导致Ca 2+信号传导减少。如果这个模型是正确的，那么PE妊娠中Ca 2+信号转导适应失败的未来治疗可以通过使用SK或IK型KCa通道激动剂来实现。在这次更新中，我们建议测试这种模型，从培养中的分离细胞到离体全血管再到全动物研究。我们将研究1）在低密度和高密度下，NP-和P-UAEC对ATP反应的膜电位和KCa电流活性的变化，以及这些变化对缝隙连接功能的依赖性，并确定拮抗剂Apamin和Tram-34或选择性激动剂NS 309对Ca ~（2+）爆发活性的影响，以表明KCa通道参与了这种反应; 2）检查这些相同的拮抗剂和激动剂对离体完整UA血管中Ca 2+、膜电位和NO产生的变化的影响; 3）确定KCa激动剂NS 309是否与ATP/ATP酶的作用相当。UTP介导妊娠期间子宫动脉血流量的NO依赖性增加，以及SK和/或IK型KCa通道介导这种作用的程度。通过这种方式，我们利用我们在妊娠血流适应机制知识方面的最新进展，现在建立了使用KCa通道激动剂在妊娠期体内最大化这种反应的有效性，特别是那些并发PE的反应。成功可能会对改善人类PE妊娠治疗的策略产生重大影响，因为这种正常的适应性反应已经失败，并且对目前的治疗具有抗药性。 公共卫生相关性：先兆子痫（PE）是一种潜在的毁灭性疾病的怀孕，可以威胁到母亲和未出生的孩子的生命。它影响了多达20%的人口。即使是轻微的PE也是危险的，可能导致早产，这会增加婴儿成年后患上多种疾病的风险，如血压，糖尿病和肥胖症。据信，在PE中，子宫动脉血管内衬的细胞不能增加它们的功能，否则会在正常妊娠中发生。我们有证据表明，正常的适应是由于电变化和他们的失败可能是“固定”与特定的药物NS 309。该项目的目的是通过动物模型测试来确定我们的怀疑是否正确。如果我们是正确的，我们可能能够提出一个治疗计划，为PE妊娠。更全面地了解细胞功能在怀孕期间是如何调节的，肯定会使我们更接近克服这种影响这个国家和其他地区许多人的潜在破坏性疾病。