THE ROLE OF TIMP-2 IN NEURAL CREST PATHFINDING

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 神经嵴细胞从胚胎神经管分离，然后在分化成许多衍生物之前在整个胚胎中广泛迁移。神经嵴细胞的一个子集，称为心脏神经嵴细胞，有助于心脏发育的几个方面，包括分隔，心肌分化和流出道的正确形成。许多先天性心脏缺陷与心脏神经嵴细胞发育异常有关。然而，很少有人知道的因素，调节心脏神经嵴迁移的胚胎。我们已经证明分泌蛋白TIMP-2由鸡心脏NC细胞表达，并且是其正常发育所必需的。该项目测试TIMP-2指导心脏NC细胞进入适当的迁移途径的假设，并将阐明它这样做的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Neural crest cells detach from the embryonic neural tube and then migrate extensively throughout the embryo before differentiating into a number of derivatives. A subset of neural crest cells, called cardiac neural crest cells, contribute to several aspects of heart development, including septation, myocardial differentiation, and proper formation of the outflow tract. Many congenital heart defects are associated with abnormal cardiac neural crest cell development. However, very little is known about the factors that regulate cardiac neural crest migration in the embryo. We have shown the secreted protein TIMP-2 is expressed by chicken cardiac NC cells and required for their normal development. This project tests the hypothesis that TIMP-2 directs cardiac NC cells into the proper migratory pathway, and will elucidate the mechanism by which it does so.