THE ROLE OF TIMP-2 IN NEURAL CREST PATHFINDING

TIMP-2 在神经嵴寻路中的作用

• 批准号: 8360015
• 负责人: MARK V REEDY
• 金额: $ 3.5万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360015
• 关键词: Cardiac; Cells; Chickens; Congenital Heart Defects; Development; Embryo; Funding; Grant; Immigration; Myocardial; National Center for Research Resources; Nebraska; Neural Crest; Neural Crest Cell; Neural tube; Pathway interactions; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Role; Source; Testing; United States National Institutes of Health; cardiogenesis; cost; functional genomics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Neural crest cells detach from the embryonic neural tube and then migrate extensively throughout the embryo before differentiating into a number of derivatives. A subset of neural crest cells, called cardiac neural crest cells, contribute to several aspects of heart development, including septation, myocardial differentiation, and proper formation of the outflow tract. Many congenital heart defects are associated with abnormal cardiac neural crest cell development. However, very little is known about the factors that regulate cardiac neural crest migration in the embryo. We have shown the secreted protein TIMP-2 is expressed by chicken cardiac NC cells and required for their normal development. This project tests the hypothesis that TIMP-2 directs cardiac NC cells into the proper migratory pathway, and will elucidate the mechanism by which it does so.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 神经脊细胞从胚胎神经管中分离出来，然后在胚胎中广泛迁移，然后分化成一些衍生细胞。神经脊细胞的一个子集，称为心脏神经脊细胞，对心脏发育的几个方面做出贡献，包括间隔、心肌分化和流出道的正常形成。许多先天性心脏病与心脏神经脊细胞发育异常有关。然而，对胚胎中调控心脏神经脊迁移的因素知之甚少。我们已经证明，分泌蛋白TIMP-2在鸡心脏NC细胞中表达，是其正常发育所必需的。该项目测试了TIMP-2引导心脏NC细胞进入正确的迁移途径的假设，并将阐明其实现这一过程的机制。