Comparative Pathology & Mouse Phenotyping

比较病理学

• 批准号: 8555660
• 负责人: KRISTA MARIE DUBRAY LA PERLE
• 金额: $ 9.89万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-12 至 2015-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8555660
• 关键词: Accounting; Address; Age; Anatomy; Animal Cancer Model; Animal Model; Animals; Antibodies; Autopsy; Biomedical Research; Blood; Breeding; Businesses; Cancer Center Support Grant; Cancer Model; Chemoprevention; Clinical; Clinical Chemistry; Clinical Pathology; Collection; Communities; Comparative Pathology; Computer software; Consultations; Data; Diagnostic radiologic examination; Doctor of Philosophy; Doctor of Veterinary Medicine; Evaluation; Experimental Designs; Experimental Pathology; Floor; Freezing; Funding; Future; Genetically Engineered Mouse; Goals; Head; Hematology; Histology; Hour; Housing; Human; Image; Immunohistochemistry; Individual; Instruction; Investigation; Laboratories; Laboratory Animals; Lesion; Light Microscope; Link; Liquid substance; Location; Malignant Neoplasms; Medical; Medicine; Memorial Sloan-Kettering Cancer Center; Microscopic; Modeling; Mus; Names; Neoplasm Metastasis; Outcome; Paraffin; Paraffin Embedding; Pathologic; Pathologist; Pathology; Phenotype; Photography; Physiology; Preparation; Publications; Recruitment Activity; Research; Research Personnel; Resource Sharing; Resources; Rodent; Safety; Scientist; Secure; Services; Site; Slide; Specimen; Sq635; Staining method; Stains; Teaching Hospitals; Telephone; Testing; Tissue Microarray; Tissues; Training and Education; Treatment Efficacy; United States National Institutes of Health; Urinalysis; Urine; Veterinary Medicine; Video Microscopy; Work; Xenograft procedure; animal model development; anticancer research; college; comparative; design; digital; experience; imaging modality; improved; member; mouse model; novel; novel therapeutics; programs; sample collection; skeletal tissue; soft tissue; therapy development; tumor

The Comparative Pathology and Mouse Phenotyping Shared Resource (CPMPSR) provides expert, readily available and affordable experimental pathology support to investigators utilizing animal models to study human cancer. Formeriy known as the Mouse Phenotyping Shared Resource (established in 2001), it has been extensively reorganized since the recruitment in September 2008 of Krista La Perle, D.V.M., Ph.D., from Memorial Sloan Kettering Cancer Center. Dr. La Perie is a board-certified veterinary pathologist and NIH-funded, postdoctoral alumna of OSU's Department of Veterinary Biosciences (VBS). The shared resource name change reflects the diverse species of animals utilized in varied projects conducted by OSUCCC members that evaluate therapeutic efficacy, chemoprevention and safety of novel therapeutics in rodent and non-rodent xenograft and metastasis models, while continuing to emphasize the phenotypic characterization of newly generated genetically engineered mice. Under Dr. La Perie's direction and strong institutional support, the laboratory was relocated, technical support was expanded, and a second, dedicated comparative pathologist is being recruited. All of these improvements are designed to improve the quality of comparative pathology support provided to OSUCCC members and address concerns of the 2004 CCSG review. Continued services include comprehensive macroscopic and microscopic examinations of various species of laboratory animals, including genetically engineered mice. Clinical pathology testing including hematology, clinical chemistry and urinalyses performed previously by the OSU Veterinary Teaching Hospital Clinical Pathology Laboratory are now conducted in-house on newly acquired analyzers by a dedicated medical laboratory technologist. The CPMPSR also utilizes reference laboratories for novel tests not performed in-house. Radiography is performed by the CPMPSR to enhance morphologic evaluations on post-mortem specimens. The CPMPSR works closely on serial, ante-mortem imaging and pathology correlates with the OSUCCC Imaging Shared Resource to assist investigators who need the wide array of ante-mortem imaging modalities offered by the OSUCCC. Preparation of routine frozen and paraffin slides, tissue microarrays and special histochemical and immunohistochemical staining previously performed by the VBS Histology and Immunohistochemistry Core is now integrated with the CPMPSR. An extensive menu of immunohistochemical antibodies specifically for use on rodent tissues has been established. Tissue microarrays incorporating tumors from various mouse models of cancer have also been prepared. The CPMPSR provides a referral service to experienced scientists within the OSU research community providing expertise in animal model development, experimental design, optimal sample collection and data interpretation. The CPMPSR is utilized by all six OSUCCC Scientific Programs and OSUCCC members account for more than 60% of all users. The CPMPSR leverages extensive institutional support, and seeks only 25.6% support from CCSG funds. The service contributes critical support to high quality cancer relevant publications and the education and training of future cancer investigators.

比较病理学和小鼠表型共享资源（CPMPSR）为利用动物模型研究人类癌症的研究人员提供专家，现成和负担得起的实验病理学支持。以前称为小鼠表型分析共享资源（成立于2001年），自2008年9月招募Krista La Perle，D.V.M.以来，已进行了广泛的重组，哲学博士、 斯隆凯特琳纪念癌症中心的La Perie博士是董事会认证的兽医病理学家和NIH资助的俄勒冈州立大学兽医生物科学系（VBS）的博士后校友。共享资源名称的变化反映了OSUCCC成员在评估新疗法的治疗效果，化学预防和安全性的各种项目中使用的不同动物物种。 啮齿动物和非啮齿动物异种移植和转移模型，同时继续强调新产生的基因工程小鼠的表型特征。在La Perie博士的指导和强有力的机构支持下，实验室搬迁，技术支持扩大，第二个专门的比较病理学家正在招聘中。所有这些改进旨在提高提供给OSUCCC成员的比较病理学支持的质量，并解决2004年CCSG审查的问题。持续的服务包括对各种实验室动物进行全面的宏观和显微镜检查，包括基因工程小鼠。以前由OSU兽医教学医院临床病理学实验室进行的临床病理学检测，包括血液学、临床化学和尿液分析，现在由专门的医学实验室技术人员在新购置的分析仪上进行。CPMPSR还利用参考实验室进行内部未进行的新检测。由CPMPSR进行X线摄影，以加强对死后标本的形态学评价。CPMPSR与OSUCCC成像共享资源密切合作，进行系列、生前成像和病理学检查，以协助需要 OSUCCC提供的多种生前成像模式。VBS组织学和免疫组织化学核心现在将常规冷冻和石蜡切片、组织微阵列以及特殊组织化学和免疫组织化学染色的制备与CPMPSR整合在一起。专门用于啮齿类动物组织的免疫组织化学抗体的广泛菜单已经公布。 确立了习还制备了掺入来自各种癌症小鼠模型的肿瘤的组织微阵列。CPMPSR为OSU研究社区内经验丰富的科学家提供转介服务，这些科学家提供动物模型开发，实验设计，最佳样品收集和数据解释方面的专业知识。CPMPSR被所有六个OSUCCC科学计划使用， OSUCCC会员占所有用户的60%以上。CPMPSR利用了广泛的机构支持，仅寻求CCSG资金的25.6%支持。该服务为高质量的癌症相关出版物以及未来癌症研究人员的教育和培训提供了关键支持。