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Comparative Pathology & Mouse Phenotyping

比较病理学

基本信息

批准号：
8601815
负责人：
KRISTA MARIE DUBRAY LA PERLE
金额：
$ 9.59万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8601815
关键词：
Accounting Address Age Anatomy Animal Cancer Model Animal Model Animals Antibodies Autopsy Biomedical Research Blood Breeding Businesses Cancer Center Support Grant Cancer Model Chemoprevention Clinical Clinical Chemistry Clinical Pathology Collection Communities Comparative Pathology Computer software Consultations Data Diagnostic radiologic examination Doctor of Philosophy Doctor of Veterinary Medicine Evaluation Experimental Designs Experimental Pathology Floor Freezing Funding Future Genetically Engineered Mouse Goals Head Hematology Histology Hour Housing Human Image Immunohistochemistry Individual Instruction Investigation Laboratories Laboratory Animals Lesion Light Microscope Link Liquid substance Location Malignant Neoplasms Medical Memorial Sloan-Kettering Cancer Center Microscopic Modeling Mus Names Neoplasm Metastasis Outcome Paraffin Paraffin Embedding Pathologic Pathologist Pathology Phenotype Photography Physiology Plasma Preparation Publications Recruitment Activity Research Research Personnel Resource Sharing Resources Rodent Safety Scientist Secure Services Site Slide Specimen Sq635 Staining method Stains Teaching Hospitals Telephone Television Testing Tissue Microarray Tissues Training and Education Treatment Efficacy United States National Institutes of Health Urinalysis Urine Veterinary Medicine Video Microscopy Work Xenograft procedure animal model development anticancer research college comparative design digital experience imaging modality improved member mouse model novel novel therapeutics programs sample collection skeletal tissue soft tissue therapy development tumor

项目摘要

The Comparative Pathology and Mouse Phenotyping Shared Resource (CPMPSR) provides expert, readily available and affordable experimental pathology support to investigators utilizing animal models to study human cancer. Formeriy known as the Mouse Phenotyping Shared Resource (established in 2001), it has been extensively reorganized since the recruitment in September 2008 of Krista La Perle, D.V.M., Ph.D., from Memorial Sloan Kettering Cancer Center. Dr. La Perie is a board-certified veterinary pathologist and NIH-funded, postdoctoral alumna of OSU's Department of Veterinary Biosciences (VBS). The shared resource name change reflects the diverse species of animals utilized in varied projects conducted by OSUCCC members that evaluate therapeutic efficacy, chemoprevention and safety of novel therapeutics in rodent and non-rodent xenograft and metastasis models, while continuing to emphasize the phenotypic characterization of newly generated genetically engineered mice. Under Dr. La Perie's direction and strong institutional support, the laboratory was relocated, technical support was expanded, and a second, dedicated comparative pathologist is being recruited. All of these improvements are designed to improve the quality of comparative pathology support provided to OSUCCC members and address concerns of the 2004 CCSG review. Continued services include comprehensive macroscopic and microscopic examinations of various species of laboratory animals, including genetically engineered mice. Clinical pathology testing including hematology, clinical chemistry and urinalyses performed previously by the OSU Veterinary Teaching Hospital Clinical Pathology Laboratory are now conducted in-house on newly acquired analyzers by a dedicated medical laboratory technologist. The CPMPSR also utilizes reference laboratories for novel tests not performed in-house. Radiography is performed by the CPMPSR to enhance morphologic evaluations on post-mortem specimens. The CPMPSR works closely on serial, ante-mortem imaging and pathology correlates with the OSUCCC Imaging Shared Resource to assist investigators who need the wide array of ante-mortem imaging modalities offered by the OSUCCC. Preparation of routine frozen and paraffin slides, tissue microarrays and special histochemical and immunohistochemical staining previously performed by the VBS Histology and Immunohistochemistry Core is now integrated with the CPMPSR. An extensive menu of immunohistochemical antibodies specifically for use on rodent tissues has been established. Tissue microarrays incorporating tumors from various mouse models of cancer have also been prepared. The CPMPSR provides a referral service to experienced scientists within the OSU research community providing expertise in animal model development, experimental design, optimal sample collection and data interpretation. The CPMPSR is utilized by all six OSUCCC Scientific Programs and OSUCCC members account for more than 60% of all users. The CPMPSR leverages extensive institutional support, and seeks only 25.6% support from CCSG funds. The service contributes critical support to high quality cancer relevant publications and the education and training of future cancer investigators.

比较病理学和小鼠表型共享资源 (CPMPSR) 为利用动物模型研究人类癌症的研究人员提供专家、现成且负担得起的实验病理学支持。以前称为小鼠表型共享资源（成立于 2001 年），自 2008 年 9 月招募 Krista La Perle（D.V.M.、Ph.D.）以来，该资源已进行了广泛的重组，来自纪念斯隆凯特琳癌症中心。 La Perie 博士是一位经过委员会认证的兽医病理学家，也是由 NIH 资助的俄勒冈州立大学兽医生物科学系 (VBS) 的博士后校友。共享资源名称的变化反映了 OSUCCC 成员开展的各种项目中使用的不同物种的动物，这些项目评估了新疗法的治疗效果、化学预防和安全性。啮齿类和非啮齿类异种移植和转移模型，同时继续强调新产生的基因工程小鼠的表型特征。在 La Perie 博士的指导和强有力的机构支持下，实验室搬迁了，技术支持扩大了，并且正在招募第二位专门的比较病理学家。所有这些改进都是为了提高向 OSUCCC 成员提供的比较病理学支持的质量，并解决 2004 年 CCSG 审查的问题。持续的服务包括对各种实验动物（包括基因工程小鼠）进行全面的宏观和微观检查。临床病理学测试（包括血液学、临床化学和尿液分析）以前由俄勒冈州立大学兽医教学医院临床病理实验室进行，现在由专门的医学实验室技术人员在新购置的分析仪上进行。 CPMPSR 还利用参考实验室进行非内部执行的新颖测试。 CPMPSR 进行射线照相，以加强对尸检标本的形态学评估。 CPMPSR 在系列、生前成像和病理学方面密切合作，与 OSUCCC 成像共享资源相关，以协助需要以下信息的研究人员： OSUCCC 提供多种死前成像模式。以前由 VBS 组织学和免疫组织化学核心执行的常规冷冻和石蜡载玻片、组织微阵列的制备以及特殊的组织化学和免疫组织化学染色现在已与 CPMPSR 集成。专门用于啮齿动物组织的大量免疫组织化学抗体已被开发出来已确立的。还制备了包含来自各种癌症小鼠模型的肿瘤的组织微阵列。 CPMPSR 为 OSU 研究界内经验丰富的科学家提供转介服务，提供动物模型开发、实验设计、最佳样本收集和数据解释方面的专业知识。 CPMPSR 被所有六个 OSUCCC 科学计划使用 OSUCCC会员占所有用户的60%以上。 CPMPSR 利用广泛的机构支持，仅寻求 CCSG 基金 25.6% 的支持。该服务为高质量的癌症相关出版物以及未来癌症研究人员的教育和培训提供了重要支持。