Comparative Pathology and Mouse Phenotyping Shared Resource

CORE-014：比较病理学和小鼠表型（CPMPSR） 项目概要/摘要 比较病理学和小鼠表型共享资源（CPMPSR）提供专家， 为利用动物模型研究人类癌症的研究人员提供可用且负担得起的病理学支持。 在癌症研究中使用动物模型，特别是近交系和基因工程小鼠， 在过去几年中，由于技术进步和 人类和小鼠基因组的测序。几乎所有OSUCCC成员都使用实验动物 模型将至少需要CPMPSR提供的一些服务。CPMPSR的具体目标 是：1）为使用各种动物种属的临床前有效性和毒性研究提供支持; 2） 为OSUCCC研究者提供咨询服务，以优化实验设计、样品提交 和数据分析，各种辅助测试所需的建议，向其他OSUCCC共享的转介 资源和核心，并转介给具有特定专业知识的研究者实验室（例如，更专业 临床病理学测试、电子显微镜检查、成像、行为和生理测试）;以及，3） 在申请资助和提交手稿时为研究人员提供咨询， 提供了方法和病理结果的细节。CPMPSR提供的服务，由Dr. Krista La Perle包括宏观和微观组织评价、临床病理学试验， 全面的组织学和免疫组织化学、显微镜载玻片成像和定量分析。的 CPMPSR还提供了基因工程小鼠的全面表型表征，包括 胚胎的详细特征。通过CPMPSR进行X线摄影，以增强形态学 对尸体样本的评估CPMPSR定期与其他OSUCCC共享 资源，如显微镜共享资源和生物医学信息学共享资源。来 在过去的资助期间，CPMPSR被OSUCCC研究致癌作用的所有五个研究项目所利用 并使用临床前模型进行药物开发。CPMPSR为117个OSUCCC成员提供了服务， 这些成员占使用量的53%。CPMPSR还支持了93种癌症的研究 出版物，其中11篇发表在影响因子> 10的期刊上。CPMPSR支持9 方案赠款（例如，P01和SPORE）。CPMPSR的未来计划是通过以下方式增加容量 增聘一名病理学家，提供更多的免疫组织化学服务， 现有软件。CPMPSR利用广泛的机构支持，仅寻求6.5%的支持， CCSG基金。比较病理学和小鼠表型共享资源是 诊断学

CORE-014: COMPARATIVE PATHOLOGY AND MOUSE PHENOTYPING (CPMPSR) PROJECT SUMMARY / ABSTRACT The Comparative Pathology and Mouse Phenotyping Shared Resource (CPMPSR) provides expert, readily available and affordable pathology support to investigators utilizing animal models to study human cancer. The use of animal models in cancer research, particularly inbred and genetically engineered mice, has increased exponentially over the past several years due to technological advances and completion of the sequencing of the human and mouse genomes. Virtually every OSUCCC member utilizing experimental animal models will need at least some of the services provided by the CPMPSR. The Specific Aims of the CPMPSR are: 1) to provide support for preclinical efficacy and toxicity studies utilizing a variety of animal species; 2) to provide consultative services to OSUCCC investigators for optimal experimental design, sample submission and data analysis, recommendations as needed for various ancillary tests, referrals to other OSUCCC shared resources and cores, and referrals to investigators' laboratories with specific expertise (e.g., more specialized clinical pathology testing, electron microscopy, imaging, behavioral and physiological testing); and, 3) to provide consultations to investigators at the time of grant applications and manuscript submissions by providing details about methods and pathologic findings. The services provided by CPMPSR, directed by Dr. Krista La Perle include macroscopic and microscopic tissue evaluations, clinical pathology testing, comprehensive histology and immunohistochemistry, microscopic slide imaging and quantitative analysis. The CPMPSR also provides comprehensive phenotypic characterization of genetically-engineered mice, including detailed characterization of embryos. Radiography is performed by the CPMPSR to enhance morphologic evaluations on post-mortem specimens. The CPMPSR regularly interacts with other OSUCCC shared resources such as the Microscopy Shared Resource and Biomedical Informatics Shared Resource. Over the past grant period, the CPMPSR was utilized by all five OSUCCC Research Programs studying carcinogenesis and using preclinical models for drug development. CPMPSR provided services to 117 OSUCCC members, and these members accounted for 53% of usage. The CPMPSR has also supported the research for 93 cancer publications, of which 11 were published in journals with an impact factor >10. The CPMPSR supported 9 programmatic grants (e.g., P01s and SPOREs). The future plans of the CPMPSR are to add capacity through the hiring of an additional pathologist, providing additional immunohistochemistry services and upgrading existing software. The CPMPSR leverages extensive institutional support, and seeks only 6.5% support from CCSG funds. The Comparative Pathology and Mouse Phenotyping Shared Resource is part of the Diagnostics Grouping.