Monkey Alcohol Tissue Research Resource (MATRR)

猴子酒精组织研究资源 (MATRR)

• 批准号: 8308542
• 负责人: KATHLEEN A GRANT
• 金额: $ 78.09万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-20 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8308542
• 关键词: Accounting; Advisory Committees; Affect; Age; Alcohol consumption; Alcoholism; Alcohols; Animal Organ; Archives; Autopsy; Bioinformatics; Biotin; CNS processing; Cercopithecus tantalus; Chronic; Communities; Data; Databases; Development; Disease; Electrophysiology (science); Endocrine; Environment; Ethanol; Freezing; Future; Genetic; Genomics; Goals; Grant; Heavy Drinking; Hepatic; Housing; Human; Instruction; Laboratories; Macaca; Macaca fascicularis; Macaca mulatta; Medical; Monkeys; Neuraxis; Organ; Pathology; Peripheral; Phenotype; Population; Principal Investigator; Procedures; Process; Proteomics; Protocols documentation; Publications; Recording of previous events; Research; Resource Informatics; Resource Sharing; Resources; Risk; Rodent; Self Administration; Self-Administered; Site; Staging; Stress; System; Techniques; Testing; Tetracyclines; Time; Tissue Banking; Tissue Banks; Tissues; alcohol consequences; alcohol content; alcohol exposure; alcohol research; base; brain behavior; cohort; data management; drinking; in vivo; interest; longitudinal design; metabolomics; neurochemistry; nonhuman primate; novel; quality assurance; sex; social; tool

DESCRIPTION (provided by applicant): Excessive alcohol ingestion, occasionally or chronically, is co-morbid with medical disorders affecting the brain and behavior as well as other organ damage. Much of what is known about risk for and the consequence of heavy alcohol consumption, including mechanisms of organ damage, is derived from rodent studies or retrospective human accounts. This application proposes establishing a unique resource for alcohol research, a Monkey Alcohol Tissue Research Resource (MATRR). From this resource both tissue and associated bioinformatics tools will be made readily available to the wider alcohol research community. The tissue is derived from a standard protocol of ethanol self-administration in 3 species of monkeys. This resource will provide novel data for hypothesis testing relating the risk for and consequences of alcohol consumption and serve to bi-directionally bridge the gap between rodent and human studies. The basis of the MATRR is that non human primates, specifically monkeys, show a range of drinking excessive amounts of alcohol (>3.0 g/kg or a 12 drink equivalent/day) over long periods of time (12-30 months) with concomitant pathological changes in endocrine, hepatic and central nervous system (CNS) processes. These longitudinal designs span "stages of drinking" from ethanol-nai've to early alcohol exposure to chronic abuse. The CNS and peripheral organs from these animals comprise a unique translational resource for mechanistic and genetic studies of ethanol-induced pathologies. The state-of-the-art necropsy protocol will provide fresh, fixed and frozen tissue that are appropriate for ex vivo electrophysiology and neurochemical recordings, histological studies and genomic, proteomic and metabolomic approaches, respectively. The demand for, and the quality of, the tissues are already high as reflected in the number of requests and publications. Nevertheless, this resource needs further development in order to fulfill its potential as a cutting edge translational tool in alcoholism research. Thus, the primary goal of this proposal is to build the resources of this tissue bank and distribute these tissues and/or associated bioinformatics, to the broader alcohol research community. RELEVANCE (See instructions): The research outlined in this proposal will establish a unique post-mortem tissue bank for alcohol research. Using sophisticated techniques tissues from populations of monkeys that have been chronically drinking alcohol and the related drinking and genetic information will be disseminated to the wider alcohol research community to better understand disease processes associated with alcoholism.

描述（由申请人提供）：偶尔或长期过量饮酒与影响大脑和行为以及其他器官损伤的医学疾病并存。关于大量饮酒的风险和后果，包括器官损伤的机制，大部分都来自啮齿动物研究或回顾性人类记录。本申请建议建立一个独特的酒精研究资源，猴子酒精组织研究资源（MATRR）。从这个资源组织和相关的生物信息学工具将随时提供给更广泛的酒精研究界。该组织来源于3种猴中乙醇自我给药的标准方案。该资源将为与饮酒风险和后果相关的假设检验提供新数据，并有助于双向弥合啮齿动物和人类研究之间的差距。MATRR的基础是，非人灵长类动物，特别是猴子，在很长一段时间（12-30个月）内表现出一定范围的过量饮酒（>3.0 g/kg或12饮酒当量/天），伴随着内分泌、肝脏和中枢神经系统（CNS）过程的病理变化。这些纵向设计跨越了“饮酒阶段”，从酒精初体验到早期酒精暴露，再到慢性滥用。这些动物的中枢神经系统和外周器官为乙醇诱导的病理学机制和遗传学研究提供了独特的翻译资源。最先进的尸检方案将提供分别适用于离体电生理学和神经化学记录、组织学研究以及基因组学、蛋白质组学和代谢组学方法的新鲜、固定和冷冻组织。对这些组织的需求和质量已经很高，这反映在要求和出版物的数量上。然而，这一资源需要进一步开发，以实现其作为酗酒研究的尖端翻译工具的潜力。因此，该提案的主要目标是建立该组织库的资源，并将这些组织和/或相关的生物信息学分发给更广泛的酒精研究界。相关性（见说明）：本提案中概述的研究将为酒精研究建立一个独特的死后组织库。利用先进的技术，长期饮酒的猴子群体的组织以及相关的饮酒和遗传信息将传播给更广泛的酒精研究界，以更好地了解与酒精中毒有关的疾病过程。