Tracking stem cells in engineered tissue and organs in vivo and in real time

体内实时追踪工程组织和器官中的干细胞

• 批准号: 8319267
• 负责人: JEREMY J MAO
• 金额: $ 54.78万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8319267
• 关键词: Adherent Culture; Adipocytes; Apoptosis; Arthritis; Back; Behavior; Biocompatible Materials; Biological Assay; Biomedical Engineering; Cardiac; Cell Proliferation; Cell Survival; Cell Transplants; Cells; Chondrocytes; Data; Defect; Development; Developmental Biology; Dyes; Encapsulated; Engineering; Goals; Healed; Home environment; Human; Human Activities; Hydrogels; Image; Implant; In Vitro; Joints; Label; Lead; Ligaments; Mechanics; Mesenchymal Stem Cells; Metabolism; Methods; Microscopic; Modality; Molecular; Mus; Muscle; Musculoskeletal; Natural regeneration; Neurons; Nude Rats; Organ; Osteoblasts; Outcome Assessment; Pancreas; Photobleaching; Population; Proliferating; Proteins; Quantum Dots; Rattus; Regenerative Medicine; Relative (related person); SCID Mice; Signal Transduction; Stem cells; System; Tail; Technology; Tendon structure; Testing; Time; Tissue Engineering; Tissues; Veins; Wound Healing; bone; cytotoxicity; healing; improved; in vivo; osteochondral tissue; public health relevance; response; scaffold; tissue regeneration; tool

DESCRIPTION (provided by applicant): In response to PAR-06-504 "Enabling Technologies for Tissue Engineering and Regenerative Medicine," we present an enabling technology of tracking stem cells with bioconjugated quantum dots (QDs) by non-destructive and non- invasive imaging, both in vivo and in real time. A common challenge in tissue regeneration, similar to developmental biology, is the tracking of cells and tissues that are developing in real time. Our preliminary data demonstrate that bioconjugated QDs are safe to label human mesenchymal stem cells (hMSCs) during proliferation for several passages, as well as differentiation into chondrocytes and osteoblasts. We also show that QD-labeled hMSCs, injected via the tail vein of SCID mice, can be tracked in vivo and in real-time by a whole body imager system. Furthermore, we were able to track multiplexing QD- labeled hMSCs injected subcutaneously in vivo and in real time. Theses findings lead to our overall hypothesis that stem cells delivered systemically home to local defects and participate in defect healing, which has been difficult to study previously due to the lack of in vivo and real time cell tracking modalities. The overall goal of this proposal is to determine the efficacy of QD labeling and tracking of MSCs in vivo and in real time, and to improve our understanding of the relative contribution of different cell populations to the healing of tissue defect. Our long-term goal is to apply QD labeling for cell tracking as a platform technology for the healing of other tissues such as cardiac, neuronal, and pancreatic, as well as other musculoskeletal tissues such as bone-ligament interface, muscle-tendon junction, etc. Public Health Relevance Statement (provided by applicant): This project has the potential to demonstrate a viable method for labeling stem cells and tracking the development of regenerating tissue in vivo and in real time. It also has a potential to contribute to our understanding of the engineered osteochondral interface that is of critical value in bioengineered replacement of arthritic joints.

描述（由申请人提供）：

项目成果

Regenerative endodontics: barriers and strategies for clinical translation.

• DOI: 10.1016/j.cden.2012.05.005
• 发表时间: 2012-07
• 期刊: Dental clinics of North America
• 作者: Mao JJ;Kim SG;Zhou J;Ye L;Cho S;Suzuki T;Fu SY;Yang R;Zhou X
• 通讯作者: Zhou X

Musculoskeletal tissue engineering by endogenous stem/progenitor cells.

内源干/祖细胞的肌肉骨骼组织工程。

• DOI: 10.1007/s00441-012-1339-2
• 发表时间: 2012
• 期刊: Cell and tissue research
• 影响因子: 3.6
• 作者: Nie,Hemin;Lee,ChangHun;Tan,Jiali;Lu,Chuanyong;Mendelson,Avital;Chen,Mo;Embree,MildredC;Kong,Kimi;Shah,Bhranti;Wang,Shuang;Cho,Shoko;Mao,JeremyJ
• 通讯作者: Mao,JeremyJ