Integrated Genetic and Genomic Resources for a Model System

模型系统的综合遗传和基因组资源

• 批准号: 8258726
• 负责人: NELSON B. FREIMER
• 金额: $ 74.12万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8258726
• 关键词: AIDS/HIV problem; Abbreviations; Advisory Committees; African; American; Award; BAC (bacterial artificial chromosome); Base Sequence; Biological; Biological Models; Biomedical Research; California; Canada; Caribbean region; Cell Line; Cells; Cercopithecidae; Cercopithecus pygerythrus; Cercopithecus tantalus; Cerebrospinal Fluid; Chromosome Mapping; Chromosome Structures; Communities; DNA Sequence Rearrangement; Data; Data Set; Development; Diabetes Mellitus; Diet; Disease; Equilibrium; Evaluation; Evolution; Fluorescent in Situ Hybridization; Founder Generation; Funding; Funding Agency; Gene Expression; Gene Expression Profile; Gene Frequency; Generations; Genes; Genetic; Genetic Materials; Genetic Polymorphism; Genome; Genomics; Genotype; HIV; Homovanillic Acid; Human; Immune; Immunity; Immunologic Deficiency Syndromes; Immunologist; Individual; Infection; Information Systems; Internet; Investigation; Karyotype; Lead; Letters; Link; Linkage Disequilibrium; Macaca; Macaca mulatta; Magnetic Resonance Imaging; Maps; Medical; Mental disorders; Metabolism; Microsatellite Repeats; Minor; Modeling; Molecular Profiling; National Center for Research Resources; National Human Genome Research Institute; Neuraxis; Neurosciences; Nomenclature; Non-Insulin-Dependent Diabetes Mellitus; Online Systems; Paper; Papio; Pattern; Population; Population Genetics; Prevention; Quantitative Trait Loci; Quebec; Recording of previous events; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; SIV; Sampling; Science; Scientist; Single Nucleotide Polymorphism; Site; South Africa; Spectral Karyotyping; Sum; Synteny; System; Taxonomy; Tissues; United States National Institutes of Health; Universities; Update; Variant; Virus; Washington; brain tissue; density; genetic association; genetic profiling; genome sequencing; genome wide association study; genome-wide; interest; lymphoblast; meetings; nonhuman primate; public health relevance; tool; trait; vervet; web site

DESCRIPTION (provided by applicant): This is the renewal of a project that is developing an integrated genetic and genomic resource for investigations of the vervet monkey (Chlorocebus aethiops), also known as the African Green Monkey. The vervet is one of the important non human primate (NHP) biomedical models, particularly for investigations of the central nervous system (CNS), metabolism, and immunity. Its value to immunologists derives largely from its status as the most abundant natural host of simian immunodeficiency virus (SIV). It is hoped that identifying the host genetic factors that protect the vervet from developing immunodeficiency disease upon infection with SIV will provide important clues for the prevention and treatment of HIV. Progress in the current award included the completion of a vervet genetic map consisting of microsatellite markers, the identification of large numbers of single nucleotide polymorphisms (SNPs) in targeted genome regions, the generation of an initial resource for vervet gene expression studies, and creating a website to provide online data access to the scientific community. The vervet genome sequencing project (VGSP) is now underway and will provide, in addition to a reference sequence, a reference vervet transcriptome for several brain tissues and immune cells, and genomewide information on common variation across the major C. aethiops subspecies. The renewal will use this information to verify genomic rearrangements, and generate a genomewide SNP array. By assembling and then genotyping using this array, a large sample of wild-caught vervet monkeys, we will carry out proof-of- concept genomewide association studies of several heritable traits. We will also extend the transcriptome resource through evaluation, in the same tissues, of inter-individual variation in expression. All of the information generated through this project will be made available to the scientific community through an integrated, regularly updated website that will also provide links to VGSP data and to other vervet and NHP projects. PUBLIC HEALTH RELEVANCE: This project will develop research tools which will facilitate a wide range of different investigations that could advance our understanding of a number of devastating common diseases. Notable examples include HIV/AIDS, mental disorders, and diabetes. These tools will allow researchers to uncover specific alterations in the genome - the sum of genetic material - that predispose towards or protect against such diseases.

描述（由申请人提供）：这是一个正在开发综合遗传和基因组资源的项目，以研究Vervet Monkey（Chlorocebus aethiops），也称为非洲绿色猴子。恶心是重要的非人类灵长类动物（NHP）生物医学模型之一，特别是用于研究中枢神经系统（CNS），代谢和免疫力。它对免疫学家的价值在很大程度上源自其作为最丰富的邻氨酸免疫缺陷病毒（SIV）的最丰富宿主的地位。希望确定保护恶心后免受免疫缺陷疾病感染后的宿主遗传因素，将为预防和治疗HIV提供重要的线索。当前奖励的进展包括完成由微卫星标记组成的Vervet遗传图，在靶向基因组区域中鉴定大量的单核苷酸多态性（SNP），生成了Vervet基因表达研究的初始资源，并创建了一个网站以提供在线数据访问科学界。 Vervet基因组测序项目（VGSP）现在正在进行中，除参考序列外，还将提供几个脑组织和免疫细胞的参考文献转录组，以及有关跨基因组范围的大型Aethiops C. aethiops subspecies的常见变化的信息。续订将使用此信息来验证基因组重排，并生成全基因组的SNP阵列。通过使用此阵列组装然后进行基因分型，这是大量野生捕获的虫猴子样本，我们将对几种可遗传性状进行全基因组的关联证明。我们还将通过在同一组织中评估表达之间的个体变化来扩展转录组资源。通过该项目生成的所有信息将通过一个定期更新的网站提供给科学界，该网站还将提供指向VGSP数据以及其他Vervet和NHP项目的链接。 公共卫生相关性：该项目将开发研究工具，这些工具将促进各种不同的研究，以提高我们对许多破坏性常见疾病的理解。值得注意的例子包括艾滋病毒/艾滋病，精神障碍和糖尿病。这些工具将使研究人员能够发现易感或预防此类疾病的基因组中的特定变化 - 遗传物质的总和。

项目成果

The static allometry of sexual and non-sexual traits in vervet monkeys.

黑长尾猴的性状和非性状的静态异速生长。

• DOI: 10.1111/bij.12440
• 发表时间: 2015
• 期刊: Biological journal of the Linnean Society. Linnean Society of London
• 作者: Rodríguez,RafaelL;Cramer,JenniferDanzy;Schmitt,ChristopherA;Gaetano,TeganJ;Grobler,JPaul;Freimer,NelsonB;Turner,TrudyR
• 通讯作者: Turner,TrudyR