MAPPING OF IMMUNODOMINANT CD8+ AND CD4+ T LYMPHOCYTE EPITOPES IN RHESUS CMV

恒河猴 CMV 免疫显性 CD8 和 CD4 T 淋巴细胞表位作图

• 批准号: 8357907
• 负责人: Amitinder Kaur
• 金额: $ 19.39万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357907
• 关键词: Acquired Immunodeficiency Syndrome; Biological Assay; CD4 Positive T Lymphocytes; CD8B1 gene; Cellular Immunity; Cytomegalovirus; Disease; Epitopes; Evaluation; Funding; Grant; Interferon Type II; Macaca mulatta; Maps; National Center for Research Resources; New England; Prevention strategy; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Source; Staining method; Stains; T-Lymphocyte Epitopes; Techniques; United States National Institutes of Health; Vaccines; base; cost; cytokine; design; enzyme linked immunospot assay

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In order to conduct a comprehensive evaluation of CMV-specific cellular immunity in rhesus macaques, immunodominant CD8-positive and CD4-positive T lymphocyte epitopes in rhesus CMV are being mapped using a variety of techniques including interferon-gamma ELISPOT assays, intracellular cytokine staining assay and tetramers. These studies will enable more precise assessment of the protective correlates of CMV-specific cellular immunity and help in the design of appropriate vaccine strategies for prevention of CMV disease. AIDS related.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 为了对恒河猴CMV特异性细胞免疫进行全面评价，正在使用多种技术，包括干扰素-γ ELISPOT测定、细胞内细胞因子染色测定和四聚体，对恒河猴CMV中的免疫显性CD 8阳性和CD 4阳性T淋巴细胞表位进行定位。这些研究将能够更精确地评估CMV特异性细胞免疫的保护相关性，并有助于设计预防CMV疾病的适当疫苗策略。 艾滋病相关