IN VIVO EVALUATION OF PRIMATE LENTIVIRUSES

灵长类慢病毒的体内评估

• 批准号: 8357593
• 负责人: DAVID M ANDERSON
• 金额: $ 37.79万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357593
• 关键词: Cells; Chemokine (C-C Motif) Receptor 5; Chinese People; Dose; Evaluation; Funding; Future; Grant; HIV-1; Human; Immune response; Infection; Intestines; Macaca; Macaca mulatta; Measures; Molecular Cloning; National Center for Research Resources; Pathogenicity; Peripheral; Peripheral Blood Mononuclear Cell; Primate Lentiviruses; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Route; Source; Subfamily lentivirinae; T-Lymphocyte; Testing; Therapeutic; United States National Institutes of Health; Vaccines; Virus; cost; design; env Genes; in vivo; lymph nodes; microbicide; nonhuman primate; simian human immunodeficiency virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. When strains of lentiviruses become available, it is important to determine the infectivity, pathogenicity, and minimal infectious dose of each virus in a particular nonhuman primate species before it can be used in vaccine trails or therapeutic testing. This project is designed to allow lentiviruses to be tested in vivo. Simian-Human Immunodeficiency Virus-SF162P4 (SHIV SF162P4) is derived from a molecular clone, SHIV SF162, a chimeric virus that contains the env gene of a CCR-5-using primary isolate of subtype B HIV-1. A macaque-passaged stock, SHIV SF162P3 was found to cause a dramatic loss of intestinal T cells followed by a gradual depletion of peripheral T cells. Lymph node cells and PBMC from a macaque infected with the SHIV SF162P3 virus two weeks after infection were amplified in human PBMC to generate a P4 stock virus and a challenge stock was prepared. The current study was undertaken to measure the infectivity and inductive ability of B- and T-cell immune responses by the intravaginal route so that these SHIVs may serve as challenge viruses in future vaccine studies and microbicide trials in Chinese rhesus macaques utilizing this route of infection.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 当慢病毒的菌株可用时，重要的是要确定特定非人类灵长类动物中每种病毒的感染性，致病性和最小感染剂量，然后才能将其用于疫苗径迹或治疗性测试。 该项目旨在使慢病毒在体内进行测试。 Simian-Human免疫缺陷病毒-SF162P4（SHIV SF162P4）源自分子克隆SHIV SF162，SHIV SF162是一种嵌合物，该病毒含有CCR-5-使用CCR-5-使用亚型B HIV-1的ccr-5-替代基因的ENV基因。 发现了猕猴的储备SHIV SF162P3导致肠道T细胞的急剧丧失，然后逐渐消耗周围T细胞。 感染后两周感染了感染SHIV SF162P3病毒的猕猴的淋巴结细胞和PBMC在人类PBMC中扩增以产生P4库存病毒，并制备了挑战量。 进行了当前的研究，以测量阴道内途径B-和T细胞免疫检查的感染和诱导能力，以便在未来的疫苗研究和中国恒河猴中使用这种感染途径在中国恒河猕猴中作为挑战病毒。