Identification of bacteria-associated cues that regulate hatching of Aedes aegypt
鉴定调节埃及伊蚊孵化的细菌相关线索
基本信息
- 批准号:8243909
- 负责人:
- 金额:$ 22.35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-03-01 至 2014-02-28
- 项目状态:已结题
- 来源:
- 关键词:AedesAgeAreaBacteriaBiologicalBiological AssayBiologyChemical StructureChemicalsCoupledCuesCulicidaeDataDengueDengue Hemorrhagic FeverDengue VirusDepositionDesiccationDevelopmentDiseaseEmbryoEventExhibitsFemaleFractionationFutureGene ExpressionGene Expression ProfileGeneticGoalsGrowthHabitatsHigh Pressure Liquid ChromatographyHumanHuman VirusInfectionInfusion proceduresInsecticidesInternationalInvestigationJuvenile HormonesLarvaLeadLifeMass FragmentographyMethodsMicrobeMosquito ControlOvipositionOxygenPatient currently pregnantPhysiologicalPlantsPublic HealthReagentResearchResistanceSemiochemicalsSensory PhysiologySiteStagingStructureSurfaceTaxonTechniquesTimeTravelVirusVirus DiseasesWaterYellow Feverbaseeggenvironmental changehatchinginnovationmicrobialneonatenovelpreventprogramsresponsesolvent extractiontoolvector
项目摘要
DESCRIPTION (provided by applicant): The mosquito Aedes aegypti is the primary vector of human viruses causing dengue and yellow fever, diseases of global public health importance. Aedes aegypti is a peri-domestic species that lays desiccation- resistant eggs in water-filled human-made containers. In the proposed research, we will conduct a comprehensive investigation of the interaction of bacteria and Ae. aegypti eggs. The main goal of the proposed project is to identify two groups of bacteria-produced chemical cues, one that stimulates and another that inhibits egg hatching. Our hypothesis, based on extensive preliminary results, that bacteria-associated chemical cues regulate hatching is a departure from the generally accepted paradigm that egg hatching is stimulated by low dissolved oxygen (DO) concentration resulting from rapid bacterial growth. Our preliminary data show that a 1-day old mixed culture of 14 bacterial species, originally isolated from an experimental mosquito habitat, stimulates egg hatching under both high and low DO conditions. However, under supposedly stimulatory low DO conditions, eggs fail to hatch when submerged in an 8-day old culture of the same mix of bacterial species, suggesting that the same bacteria species can produce stimulatory and inhibitory cues, depending on the age of culture. In the proposed research, we will screen 14 bacterial species isolates from experimental mosquito habitats for bioactivity in stimulating and inhibiting egg hatch. Bacterial species exhibiting highly stimulatory or inhibitory bioactivity will be selected for further research. Chemical compounds associated with bacterial species will be identified by using bioassay-guided solvent extraction and fractionation coupled with chemical analytical techniques, including high-performance liquid chromatography and gas chromatography, and mass spectrometry. The activity of putative bioactive compounds will be verified in egg hatching bioassays. Once identified, the cue(s) that control hatching may be used to disrupt hatching of the pharate larva from the mosquito egg and offer innovative tools for control of a virus vector of global importance. The proposed research will provide a more complete understanding of the strategies that Ae. aegypti eggs employ to maximize survival under changing environmental conditions. Moreover, the project will provide tools and reagents for future research on the physiological events during the transition from embryo to neonate, including gene expression patterns and studies of sensory physiology.
PUBLIC HEALTH RELEVANCE: Aedes aegypti is vector for viruses that cause dengue and yellow fever, diseases of worldwide importance. Discovery of bacteria-associated factors that regulate hatching may lead to the development of novel, biologically-based tools for managing this important vector species.
描述(由申请方提供):埃及伊蚊是引起登革热和黄热病(具有全球公共卫生重要性的疾病)的人类病毒的主要载体。埃及伊蚊是一种生活在家庭附近的物种,它在充满水的人造容器中产卵,耐干燥。在拟议的研究中,我们将进行细菌和Ae的相互作用的全面调查。埃及卵该项目的主要目标是确定两组细菌产生的化学线索,一组刺激,另一组抑制鸡蛋孵化。我们的假设,广泛的初步结果的基础上,细菌相关的化学线索调节孵化是一个偏离普遍接受的范例,鸡蛋孵化刺激低溶解氧(DO)浓度导致细菌快速生长。我们的初步数据显示,最初从实验蚊子栖息地分离的14种细菌的1天大混合培养物在高DO和低DO条件下都会刺激卵子孵化。然而,在假定的刺激性低DO条件下,当将卵浸没在相同细菌种类混合物的8天培养物中时,卵未能孵化,这表明相同细菌种类可以产生刺激性和抑制性线索,这取决于培养物的年龄。在这项研究中,我们将从实验蚊子栖息地筛选14种细菌分离物,以研究其刺激和抑制卵孵化的生物活性。将选择表现出高度刺激或抑制生物活性的细菌物种用于进一步研究。将通过使用生物测定指导的溶剂提取和分馏,结合化学分析技术,包括高效液相色谱法和气相色谱法,以及质谱法,鉴定与细菌物种相关的化合物。推定的生物活性化合物的活性将在卵孵化生物测定中验证。一旦鉴定,控制孵化的线索可用于破坏从蚊子卵孵化出的噬菌体幼虫,并提供用于控制全球重要病毒载体的创新工具。拟议的研究将提供一个更完整的了解的战略,Ae。埃及卵用来在不断变化的环境条件下最大限度地生存。此外,该项目将为未来研究从胚胎到新生儿过渡期间的生理事件提供工具和试剂,包括基因表达模式和感觉生理学研究。
公共卫生相关性:埃及伊蚊是引起登革热和黄热病的病毒的媒介,这些疾病在世界范围内都很重要。发现细菌相关的因素,调节孵化可能会导致新的,生物为基础的工具,管理这一重要的载体物种的发展。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Loganathan Ponnusamy其他文献
Loganathan Ponnusamy的其他文献
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First detection of rickettsiae in a US chigger population and the potential risk to people
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$ 22.35万 - 项目类别:
Identification of bacteria-associated cues that regulate hatching of Aedes aegypt
鉴定调节埃及伊蚊孵化的细菌相关线索
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8427270 - 财政年份:2012
- 资助金额:
$ 22.35万 - 项目类别:
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