Animal care: supporting research on pathogenesis and treatment of autoimmunity

动物护理：支持自身免疫发病机制和治疗的研究

• 批准号: 8158460
• 负责人: Massimo Gadina
• 金额: $ 111.5万
• 依托单位: NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8158460
• 关键词: Autoimmunity; Pathogenesis; Research Support; animal care

The LACU assists the NIAMS IRP investigators with their research involving animals. Such animals are housed in shared animal facilities on the NIH campus including the NIAMS LACU directly overseen facility located in Building 10 in 9/C127 in the ACRF Buildings 6B, 10A, 50 and the Building 14 complex. NIAMS animal users work primarily with mice and rats. The NIAMS IRP Animal Program has oversight for 20 Animal Study Proposals (ASPs) using mouse and rat models. The LACU assists researchers prepare ASPs and has 3 voting members and 1 alternate on the NIAMS Animal Care and Use Committee (ACUC) and maintains the foster colony and the sentinel program for the IRP. Projects include arthritis, allergic rhinitis and other transmit receptor-induced cellular responses, recombination/chromatin remodeling using AID transgenic mice, familial Mediterranean fever (FMF), and TNF receptor associated periodic syndrome. Research involves muscle and related diseases (experimentation with: N-RAP protein, glycogen storage disease Type II, SIRT1 gene, myofibril assembly, and myositis) and sub-cellular organelles and cytoskeleton in skeletal muscle as well as in vitro experiments examining retroviral transduction, bone marrow transplantation, and epidermal differentiation using fetal and adult skin tissues. Many of the institutes studies involve apoptosis and autoimmunity, as well as cartilage studies focused on tissue-engineered cartilage, chondrogenesis, GDF-F, COMP and osteoarthritis. The NIAMS IRP LACU facilitated the accreditation of NIH with the Association for the Assessment and Accreditation of Laboratory Animal Care International (AALACi) during the program review and inspection in 2007, the next inspection will be in early summer of 2011. A proposal to renovate the 10/9C127 Animal Facility has been accepted and the physical renovation will start soon. This will add 720 additional mouse cages to the existing facility.

LACU协助NIAMS IRP研究人员进行涉及动物的研究。这些动物饲养在NIH园区的共享动物设施中，包括位于ACRF 6 B、10A、50号楼9/C127 10号楼和14号楼综合体的NIAMS LACU直接监督设施。NIAMS动物用户主要使用小鼠和大鼠。 NIAMS IRP动物项目监督了20项使用小鼠和大鼠模型的动物研究提案（ASP）。LACU协助研究人员准备ASP，并在NIAMS动物护理和使用委员会（ACUC）中拥有3名投票成员和1名候补成员，并为IRP维护寄养群体和哨兵计划。 项目包括关节炎、过敏性鼻炎和其他传递受体诱导的细胞反应、使用AID转基因小鼠的重组/染色质重塑、家族性地中海热（FMF）和TNF受体相关的周期性综合征。研究涉及肌肉和相关疾病（实验：N-RAP蛋白，糖原累积病II型，SIRT 1基因，肌原纤维组装和肌炎）和亚细胞器和骨骼肌中的细胞骨架以及体外实验检查逆转录病毒转导，骨髓移植和使用胎儿和成人皮肤组织的表皮分化。该研究所的许多研究涉及细胞凋亡和自身免疫，以及软骨研究，重点是组织工程软骨，软骨形成，GDF-F，COMP和骨关节炎。NIAMS IRP LACU在2007年的项目审查和检查期间促进了NIH与国际实验动物评估和认证协会（AALACi）的认证，下一次检查将于2011年初夏进行。 已接受翻新10/9 C127动物设施的建议，物理翻新将很快开始。 这将在现有设施的基础上增加720个鼠笼。