Optimization of tissue depletion for LCM-MS coupling

使用最先进的质谱仪，我现在正在通过一种先进的基于蛋白质的分子图谱方法分析40个人类肿瘤样本。这项拟议工作的基本原理是，一旦使用先进的质谱学和相关的癌蛋白质组技术建立了最优的方法参数，以促进方法开发，以研究感兴趣的实体肿瘤，均质组织学肿瘤/组织细胞(即肿瘤本身、间质等)可以有效地研究生物标记物、药物靶点和途径，目的是提高我们对给定疾病状态的基本理解。

Using state-of-the-art mass spectrometry instrumentation, I am now analyzing 40 human tumor samples via an advanced protein-based molecular profiling approach. The rationale for the proposed work is that, once the optimal method parameters are established using advanced mass spectrometry and relevant oncoproteomic technologies to facilitate method development, to investigate a solid tumor of interest, homogeneous histological tumor/tissue cells (i.e., tumor proper, stroma, etc) can be effectively studied for biomarkers, drug targets, and pathways, with the goal of improving our basic understanding of a given disease-state.