Optimization of tissue depletion for LCM-MS coupling

LCM-MS 耦合的组织损耗优化

• 批准号: 8157716
• 负责人: Donald Johann
• 金额: $ 12.61万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8157716
• 关键词: Address; Arts; Biological; Coupling; Disease; Goals; Mass Spectrum Analysis; Methods; Pathway interactions; Preparation; Proteins; Signal Transduction; Solid Neoplasm; Technology; Tissues; base; improved; instrumentation; method development

To accomplish this goal, it was essential to purchase state of the art mass spectrometry instrumentation. The rationale for the proposed work is that, once the optimal method parameters are established using advanced mass spectrometry and relevant oncoproteomic technologies to facilitate method development, to investigate a solid tumor of interest, homogeneous histological tumor/tissue cells (i.e., tumor proper, stroma, etc) can be effectively studied for biomarkers, drug targets, and pathways, with the goal of improving our basic understanding of a given disease-state.

为了实现这一目标，必须购买最先进的质谱仪器。这项工作的基本原理是，一旦使用先进的质谱和相关的肿瘤蛋白质组学技术建立了最佳方法参数以促进方法开发，为了研究感兴趣的实体瘤，可以有效地研究同质组织学肿瘤/组织细胞（即肿瘤本身、基质等）的生物标志物、药物靶点和途径，目的是提高我们对给定的实体瘤的基本理解。 疾病状态。