Optimization of tissue depletion for LCM-MS coupling

为了实现这一目标，购买最先进的质谱仪是必不可少的。这项拟议工作的基本原理是，一旦使用先进的质谱学和相关的癌蛋白质组技术建立了最优的方法参数，以促进方法开发，以研究感兴趣的实体肿瘤，均质组织学肿瘤/组织细胞(即肿瘤本身、间质等)可以有效地研究生物标记物、药物靶点和途径，目的是提高我们对给定疾病状态的基本理解。

To accomplish this goal, it was essential to purchase state of the art mass spectrometry instrumentation. The rationale for the proposed work is that, once the optimal method parameters are established using advanced mass spectrometry and relevant oncoproteomic technologies to facilitate method development, to investigate a solid tumor of interest, homogeneous histological tumor/tissue cells (i.e., tumor proper, stroma, etc) can be effectively studied for biomarkers, drug targets, and pathways, with the goal of improving our basic understanding of a given disease-state.