Optimization of tissue depletion for LCM-MS coupling

LCM-MS 耦合的组织损耗优化

• 批准号: 8554720
• 负责人: Donald Johann
• 金额: $ 13.74万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8554720
• 关键词: Address; Biological; Biological Markers; Cells; Coupling; Disease; Drug Delivery Systems; Goals; Human; Immune; Mass Spectrum Analysis; Methods; Molecular Profiling; Pathway interactions; Preparation; Proteins; Proteomics; Sampling; Signal Transduction; Solid Neoplasm; Techniques; Technology; Tissues; Tumor Tissue; Work; base; improved; instrumentation; interest; method development; research study; tumor

Using state-of-the-art mass spectrometry instrumentation, I am now analyzing 40 human tumor samples via an advanced protein-based molecular profiling approach. The rationale for the proposed work is that, once the optimal method parameters are established using advanced mass spectrometry and relevant oncoproteomic technologies to facilitate method development, to investigate a solid tumor of interest, homogeneous histological tumor/tissue cells (i.e., tumor proper, stroma, etc) can be effectively studied for biomarkers, drug targets, and pathways, with the goal of improving our basic understanding of a given disease-state.

使用最先进的质谱仪器，我现在正在通过先进的基于蛋白质的分子分析方法分析40个人类肿瘤样本。 所提出的工作的基本原理是，一旦使用先进的质谱和相关的癌蛋白质组学技术建立了最佳方法参数以促进方法开发，为了研究感兴趣的实体瘤，同质组织学肿瘤/组织细胞（即，肿瘤本身、间质等）可以有效地研究生物标志物、药物靶点和途径，目的是提高我们对给定疾病状态的基本理解。

项目成果

Cancer and the tumor microenvironment: a review of an essential relationship.

• DOI: 10.1007/s00280-008-0881-9
• 发表时间: 2009-03
• 期刊: CANCER CHEMOTHERAPY AND PHARMACOLOGY
• 影响因子: 3
• 作者: Mbeunkui, Flaubert;Johann, Donald J., Jr.
• 通讯作者: Johann, Donald J., Jr.

Optimized method for computing (18)O/(16)O ratios of differentially stable-isotope labeled peptides in the context of postdigestion (18)O exchange/labeling.

• DOI: 10.1021/ac101284c
• 发表时间: 2010-07-01
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Ye, Xiaoying;Luke, Brian T.;Johann, Donald J., Jr.;Ono, Akira;Prieto, DaRue A.;Chan, King C.;Issaq, Haleem J.;Veenstra, Timothy D.;Blonder, Josip
• 通讯作者: Blonder, Josip