THE ROLES OF PROHIBITINS (PHBS) IN 3T3-L1 ADIPOCYTE DIFFERENTIATION

抑制素 (PHBS) 在 3T3-L1 脂肪细胞分化中的作用

• 批准号: 8357158
• 负责人: Dong Liu
• 金额: $ 11.87万
• 依托单位: MOREHOUSE SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-19 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357158
• 关键词: 3T3-L1 Cells; Biomedical Research; Data; Estrogen Receptors; Funding; Goals; Grant; Mus; National Center for Research Resources; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Role; Signal Pathway; Small Interfering RNA; Source; United States National Institutes of Health; adipocyte differentiation; cost; lipid biosynthesis; medical schools; multidisciplinary; prohibitin; receptor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A. Specific Aims The goal of this proposal is to determine the functional significance of prohibitins during 3T3-L1 adipocyte differentiation. Our preliminary data showed that both of these prohibitins (PHBs), including PHB1 and PHB2 (repressor of estrogen receptor activity, REA), were significantly increased during the adipocyte differentiation of 3T3-L1 preadipocytes. Moreover, 3T3-L1 adipocyte differentiation was impaired after the simultaneous knockdown of either PHBs protein by siRNA-PHB1 or siRNA-REA. So, our first aim is to determine whether PHB1 and PHB2 are critical factors in promoting mouse 3T3-L1 adipocyte differentiation. Our second aim is to investigate the signaling pathway of PHB1 and PHB2 during 3T3-L1 cell adipogenesis.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 A.具体目标 本提案的目的是确定在3 T3-L1脂肪细胞分化过程中，白藜芦醇苷的功能意义。我们的初步数据表明，这两个阿比丁（PHB），包括PHB 1和PHB 2（雌激素受体活性的阻遏物，REA），在3 T3-L1前脂肪细胞的脂肪细胞分化过程中显着增加。此外，3 T3-L1脂肪细胞分化受损后，同时敲低任一PHBs蛋白的siRNA-PHB 1或siRNA-REA。因此，我们的第一个目标是确定PHB 1和PHB 2是否是促进小鼠3 T3-L1脂肪细胞分化的关键因素。我们的第二个目的是研究PHB 1和PHB 2在3 T3-L1细胞脂肪形成过程中的信号通路。