RNAI-BASED GENE SILENCING IN NONHUMAN PRIMATES

非人类灵长类动物中基于 RNAI 的基因沉默

• 批准号: 8357953
• 负责人: Wei-Dong Yao
• 金额: $ 1.38万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357953
• 关键词: Cells; Funding; Gene Silencing; Goals; Grant; Macaca mulatta; Mediating; Mental disorders; Mitotic; National Center for Research Resources; Neurobiology; Neurons; New England; Primates; Principal Investigator; Process; Proteins; RNA Interference; Research; Research Infrastructure; Resources; Source; System; United States National Institutes of Health; Viral; base; cost; nervous system disorder; nonhuman primate; therapeutic development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. RNA interference (RNAi) is an evolutionarily conserved process of sequence-specific post-transcriptional gene silencing. Viral-mediated RNAi allows rapid knockdown of endogenous proteins in post-mitotic cells, including neurons and has great potential in facilitating understanding the neurobiological basis of and therapeutic development against many psychiatric and neurological disorders. The goal of this project is to develop a RNAi-based gene silencing system in rhesus macaque.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 RNA干扰（RNAi）是一种进化上保守的序列特异性转录后基因沉默过程。病毒介导的RNAi允许快速敲低有丝分裂后细胞（包括神经元）中的内源性蛋白，并且在促进理解许多精神和神经障碍的神经生物学基础和治疗开发方面具有巨大潜力。本项目的目标是在恒河猴中建立一个基于RNAi的基因沉默系统。