ADAPTING MICROTITERPLATE ASSAYS TO AN AUTOMATED PLATFORM

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目的目标是简化和标准化内分泌核心的常见分析分析，以便为科学家和动物管理人员提供快速、更可靠和更经济的服务。选择传统上使用微量滴定板格式的分析方法以适应自动化平台，即ACS-180。之前对雌酮结合物、孕二醇-3-葡萄糖苷、卵泡刺激素和猴绒毛膜促性腺激素的免疫测定进行了调整。在过去，促甲状腺激素、猴绒毛膜促性腺激素和非激素分析，如转铁蛋白和纤维连接蛋白也被采用。 该项目的结果是目前能够使用ACS-180自动分析仪执行所有指定的分析。它的出现将缩短周转时间，降低成本，提供更高的可靠性，并为更广泛的用户群和国家服务提供服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objective of the project is to simplify and standardize common analytical assays from the Endocrine Core in order to provide a fast, more reliable and more economical service to scientists and animal managers. Assays that have traditionally used a microtitper plate format were selected for adaptation to an automated platform, the ACS-180. Previously the immunoassays for estrone conjugates, pregnandiol-3-glucurinide, follicle stimulating hormone and monkey chorionic gonadotropin were adapted. In the past, thyroid stimulating hormone, monkey chorionic gonadotropin and non-hormone analyses such as transferrin, and fibronectin were also adapted. The result of this project is the current ability to perform all of the designated assays using the ACS-180 autoanalyzer. The advent will shorten turn-around time, reduce costs, provide increased reliability and provide service to a broader user base and a national service.