ADAPTING MICROTITERPLATE ASSAYS TO AN AUTOMATED PLATFORM

将微量滴定板测定调整至自动化平台

• 批准号: 8357262
• 负责人: Bill Lee Lasley
• 金额: $ 5.04万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357262
• 关键词: Animals; Biological Assay; California; Chorionic Gonadotropin; Endocrine; Estrone; Fibronectins; Follicle Stimulating Hormone; Funding; Grant; Immunoassay; Monkeys; National Center for Research Resources; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Scientist; Services; Source; Thyrotropin; Time; Transferrin; United States National Institutes of Health; base; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objective of the project is to simplify and standardize common analytical assays from the Endocrine Core in order to provide a fast, more reliable and more economical service to scientists and animal managers. Assays that have traditionally used a microtitper plate format were selected for adaptation to an automated platform, the ACS-180. Previously the immunoassays for estrone conjugates, pregnandiol-3-glucurinide, follicle stimulating hormone and monkey chorionic gonadotropin were adapted. In the past, thyroid stimulating hormone, monkey chorionic gonadotropin and non-hormone analyses such as transferrin, and fibronectin were also adapted. The result of this project is the current ability to perform all of the designated assays using the ACS-180 autoanalyzer. The advent will shorten turn-around time, reduce costs, provide increased reliability and provide service to a broader user base and a national service.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该项目的目标是简化和标准化内分泌核心的常见分析测定，以便为科学家和动物管理人员提供快速，更可靠和更经济的服务。选择传统上使用微量滴定板格式的测定以适应自动化平台ACS-180。以前，雌酮结合物、孕二醇-3-葡糖苷酸、促卵泡激素和猴绒毛膜促性腺激素的免疫测定法被改编。在过去，促甲状腺激素，猴绒毛膜促性腺激素和非激素分析，如转铁蛋白和纤连蛋白也进行了调整。 该项目的结果是目前能够使用ACS-180自动分析仪执行所有指定的测定。该系统的出现将缩短周转时间，降低成本，提高可靠性，并向更广泛的用户群和全国服务提供服务。