ENDOCRINE CORE SERVICES

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: The Endocrine Core has provided assays for twenty-two scientists, and recharged approximately $166,000. Our assays include routine analysis of steroid hormones in plasma, feces, urine and saliva and unique analyses that are not available elsewhere. Similarly, its assay development is directed towards providing assays that are used by our faculty and collaborators but are not readily available to them. During the past year the Endocrine Core has extended its service to include metabolic hormones and carrier proteins. It is our intent to provide in-house service for all assays requested through Therapeutics, Research Services and by individual investigators. Some of the new assays are being formatted on the ACS-180 automated chemiluminescence platform. A macaque thyroid panel as well as monkey chorionic gonadotropin (mCG) assays are now available. New cell based bioassays now provide direct measurements of circulating bioavailable estrogens, androgens and luteinizing hormone. HPLC with electromagnetic detection is being developed for assay of monoamines.