SMALL ANIMAL IMAGING

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 小动物成像核心(核心2)将为拟议项目的啮齿动物模型体内成像提供支持。高级磁共振中心和核心2的主任汤纳博士将监督体内啮齿动物成像的所有方面，涉及拟议项目中将使用的7特斯拉小动物核磁共振的使用。对于血管成像，大血管(血管造影(MRA))和小血管(DCE(动态增强)-MRI和灌注MRI)的磁共振成像可用于评估项目1和项目5的血管生成。汤纳在开发可用于评估炎症(例如iNOS)和血管生成(例如VEGF-R2)标记物的分子MRI纳米探针方面拥有丰富的经验，可用于评估项目4中的囊内iNOS水平，以及项目2和项目5中的VEGF-R3评估体内的VEGF-R3水平。这些化合物还可以通过其他成像方式进行监测，例如与Core 1合作，通过分子MRI探针上的生物素标签进行显微荧光成像。Tner博士还在开发基于氧化铁的纳米颗粒，可以在项目3中使用它来检测体内动脉粥样硬化中的巨噬细胞。最近，我们使用基于氧化铁的纳米探针来专门检测体内血管内皮生长因子-R2的水平。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Small Animal Imaging Core (Core 2) will provide support for in vivo imaging of rodent models for the Proposed Projects. Dr. Towner, the Director of the Advanced Magnetic Resonance Center, and of Core 2, will oversee all aspects of in vivo rodent imaging involving the use of the 7 Tesla small animal MRI that will be utilized in the Proposed Projects. For vascular imaging, magnetic resonance (MR) imaging on large (angiography (MRA)) and small vessels (DCE (dynamic contrast-enhanced)-MRI and perfusion MRI) can be used to assess angiogenesis for Projects 1 and 5. Towner has extensive experience in developing molecular MRI nanoprobes that can be used to assess levels of inflammatory (e.g. iNOS) and angiogenesis (e.g. VEGF-R2) markers, which can be used in Project 4 to assess intracapsular iNOS levels, and the same approach for VEGF-R3 in Projects 2 and 5 to assess in vivo VEGF-R3 levels. These compounds also have the capability of being monitored by other imaging modalities such as microscopic fluorescence imaging via a biotin tag on the molecular MRI probes in collaboration with Core 1. Dr. Towner is also developing iron oxide-based nanoparticles, which can be used to detect macrophages in vivo in atherosclerosis for Project 3. Recently we have used iron oxide-based nanoprobes to specifically detect VEGF-R2 levels in vivo.