MRI CORE

核磁共振核心

• 批准号: 7960006
• 负责人: Rheal A Towner
• 金额: $ 14.36万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960006
• 关键词: Ablation; Angiography; Animals; Bioenergetics; Biomedical Research; Brain; Brain Neoplasms; Breast; Choline; Computer Retrieval of Information on Scientific Projects Database; Contrast Media; Creatine; Development; Disease; Experimental Animal Model; Funding; Grant; Image; Institution; Investigation; Lasers; Lesion; Lipids; Liquid substance; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Magnetism; Malignant Neoplasms; Malignant neoplasm of brain; Metabolic; Methods; Microscopic; Microscopy; Modeling; Molecular; Molecular Target; Monitor; Mus; Oklahoma; Organ; Pathogenesis; Phase; Phospholipids; Rattus; Research; Research Personnel; Resolution; Resources; Rodent; Source; Spectrum Analysis; Techniques; Technology; Temperature; Therapeutic Agents; Tissues; United States National Institutes of Health; Vascular System; base; design; imaging modality; imaging probe; in vivo; instrument; magnetic field; melanoma; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. At OMRF and OUHSC we utilize high-resolution MR techniques (including microscopic imaging and spectroscopy) for biomedical research. The use of rodent experimental animal models has dramatically advanced our ability to analyze and understand the molecular basis of various diseases, however few methods exist to be able to visualize the various tissues, organs and vascular systems of rodents in vivo and non-invasively at any meaningful resolution. The following instrument, a Bruker Biospin Biospec 70/30 USR horizontal magnetic small animal imaging spectrometer, is capable of obtaining in vivo microimages (at ~100 ¿m resolution or better for microscopic MRI) in rodent experimental animal models, and in addition provide in vivo functional information on molecular targets (contrast-enhanced MRI) as well as structural information on metabolites, such as lipids/phospholipids, bioenergetic compounds, creatine, choline, and lactate (using magnetic resonance spectroscopy (MRS)). Magnetic resonance imaging (MRI), and its related techniques in biomedical research, such as MR angiography (MRA), MR spectroscopy (MRS), have developed over the past few years with significant advances in high-field magnets, high-strength magnetic field gradients, imaging probe designs and tissue-specific contrast agents, which have allowed selective morphological, functional and metabolic investigations in mice and rats to be possible. Another recent advancement in MR technology has been the development of MR microscopy. Images can be obtained with in-plane resolutions better than 100 micron, and in-vivo spectra can be collected with sensitivity and spectral resolution previously obtained only by narrow-bore liquid NMR spectrometers. Morphological MRI and/or microscopic MRI (~100 ¿m resolution) is currently used in our facility to localize and determine the extent of brain cancer lesions in a rat model (Towner) and breast and/or melanoma cancer lesions in rats and mice (Chen), and to monitor temperature changes in laser-induced tumor ablation with the use of phase-sensitive and chemicla-shift imaging (CSI) methods(Chen). Magnetic resonance spectroscopy (MRS), using image-guided MRS, is also used to monitor metabolic profiles for rat brain tumor pathogenesis (lipid and brain metabolite alterations; Towner), as well as assess therapeutic agents (Towner).

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在OMRF和OUHSC，我们利用高分辨率MR技术（包括显微成像和光谱学）进行生物医学研究。 啮齿动物实验动物模型的使用极大地提高了我们分析和理解各种疾病的分子基础的能力，然而很少有方法能够以任何有意义的分辨率在体内和非侵入性地可视化啮齿动物的各种组织、器官和血管系统。 以下仪器，Bruker Biospin Biospec 70/30 USR水平磁性小动物成像光谱仪，能够获得体内显微图像（约100分）m分辨率或更好的显微MRI）在啮齿动物实验动物模型中，此外还提供有关分子靶点的体内功能信息（对比增强MRI）以及代谢物的结构信息，例如脂质/磷脂、生物能量化合物、肌酸、胆碱和乳酸盐（使用磁共振波谱（MRS））。 磁共振成像（MRI）及其在生物医学研究中的相关技术，如磁共振血管造影（MRA）、磁共振波谱（MRS），在过去几年中随着高场磁体、高强度磁场梯度、成像探头设计和组织特异性造影剂的显著进步而发展，这使得在小鼠和大鼠中进行选择性形态学、功能和代谢研究成为可能。 MR技术的另一个最新进展是MR显微镜的发展。 图像可以以优于100微米的面内分辨率获得，并且可以以先前仅通过窄孔液体NMR光谱仪获得的灵敏度和光谱分辨率收集体内光谱。形态MRI和/或显微镜MRI（~100 μ m分辨率）目前在我们的设施中用于定位和确定大鼠模型（托纳）中脑癌病变的程度以及大鼠和小鼠中乳腺癌和/或黑色素瘤病变（Chen），并使用相敏和化学位移成像（CSI）方法监测激光诱导肿瘤消融中的温度变化（Chen）。 使用图像引导MRS的磁共振波谱（MRS）也用于监测大鼠脑肿瘤发病机制的代谢特征（脂质和脑代谢物改变;托纳），以及评估治疗剂（托纳）。