MR HISTOLOGY OF THE ADULT RAT, COMPARING IN VIVO AND EX VIVO IMAGES

成年大鼠的 MR 组织学，体内和离体图像的比较

• 批准号: 8363199
• 负责人: FULTON T CREWS
• 金额: $ 0.31万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363199
• 关键词: Adult; Animals; Brain region; Funding; Grant; Head; Histology; Image; Longitudinal Studies; Magnetic Resonance; Measures; Methods; Microscopy; National Center for Research Resources; Neurologic; Perfusion; Principal Investigator; Protocols documentation; Rattus; Relative (related person); Reproducibility; Research; Research Infrastructure; Resolution; Resources; Scanning; Source; Time; Translating; United States National Institutes of Health; Wistar Rats; alcohol exposure; cost; expectation; in vivo; interest; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Magnetic resonance histology has become an important tool for quantitative volume changes in neurologic studies. We are particularly interested in changes in specific regions of the brain of animals (Wistar rats) exposed to alcohol. We are currently performing longitudinal studies at the BRIC at UNC to follow such changes. Part of our protocol involves end point ex vivo (MR Histology) to determine the relative accuracy and reproducibility (in vivo vs. ex vivo). Reviewers have raised questions over the validity of inferring volumes measured in ex vivo to the in vivo case. Our expectation is that the ex vivo studies will be far more accurate measures of volumes. MR Histology has not been as fully developed at the BRIC as it has at the CIVM. We would seek the CIVM's help in translating the perfusion and scanning protocols. At the same time we would like to understand the relevant scanning methods. Animals supplied by: Perfusion-fixed rat heads will be prepared at UNC from animals that have been scanned (both in vivo and ex vivo) at the BRIC. CIVM will provide high-resolution MR Histology to match the studies performed at UNC.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 磁共振组织学已成为神经学研究中定量体积变化的重要工具。我们特别感兴趣的是暴露于酒精的动物（Wistar大鼠）大脑特定区域的变化。我们目前正在金砖四国进行纵向研究，以跟踪这些变化。我们的方案的一部分涉及终点离体（MR组织学），以确定相对准确性和再现性（体内与离体）。评审员对将离体测量的体积推断为体内情况的有效性提出了疑问。我们的期望是离体研究将是更准确的体积测量。在金砖四国，磁共振组织学还没有像在CIVM那样得到充分发展。我们会寻求CIVM的帮助来翻译灌注和扫描协议。同时我们也想了解一下相关的扫描方法。 动物供应商：将在2013年从在BRIC扫描（体内和离体）的动物中制备灌注固定大鼠头。CIVM将提供高分辨率MR组织学检查，以匹配在CIMB进行的研究。